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- W1979136410 abstract "A conventional two-step freezing procedure was developed and optimized in order to cryopreserve oyster embryos. The effects of cooling rate, choice of cryoprotectant, and seeding temperature on the survival of late-stage oyster embryos were examined. When these factors were optimized, improved survival rates of 78 +/- 8 and 83 +/- 7% were achieved using 2 M Me(2)SO or glycerol, respectively, as the cryoprotectant. The experimental results indicate that oyster embryos survive after freezing over a broad range of cooling rates ranging from -0.5 to -16 degrees C/min. Me(2)SO, glycerol, propylene glycol, and ethylene glycol may be used as cryoprotectants for the cryopreservation of oyster embryos. Copyright 1999 Academic Press." @default.
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- W1979136410 date "1999-09-01" @default.
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- W1979136410 title "Factors Affecting Survival of Cryopreserved Oyster (Crassostrea gigas) Embryos" @default.
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- W1979136410 doi "https://doi.org/10.1006/cryo.1999.2196" @default.
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