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• W1979136645 abstract "Intracellular antibodies or intrabodies have great potential in protein knockout strategies for intracellular antigens. We applied the Intracellular Antibody Capture Technology for the direct selection in yeast of a mouse scFv library (V(L)-V(H) format) constructed from animals immunised with recombinant non-structural protein NSP5 of Rotavirus. We selected five different intracellular antibodies (ICAbs), which specifically recognize Delta2, an NSP5 deletion mutant used as bait. The anti-NSP5 ICAbs were well expressed both in yeast and mammalian cells as cytoplasmic or nuclear-tagged forms. By immunofluorescence and co-immunoprecipitation assays we characterised the intracellular interaction of the five anti-NSP5 ICAbs with the co-expressed antigens." @default.
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• W1979136645 date "2005-06-01" @default.
• W1979136645 modified "2023-09-26" @default.
• W1979136645 title "Design and selection of an intrabody library produced de-novo for the non-structural protein NSP5 of rotavirus" @default.
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• W1979136645 doi "https://doi.org/10.1016/j.jim.2005.03.011" @default.
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