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- W1979183998 abstract "It has been brought to the author's attention that the above paper containsmissing text in the Abstract and they would like to correct this. The text was inadvertently deleted during the typesetting and editing of the proofs. On page 166, the Abstract should read as follows: Eukaryotes have evolved complex cellular responses to double-stranded RNA. One response that is highly conserved across many species is the RNA silencing pathway. Tombusviruses have evolved a mechanism to evade the RNA silencing pathway that involves a small protein, p19, that acts as a suppressor of RNA silencing. This protein binds specifically to small-interfering RNAs (siRNAs) with nanomolar affinity in a sequence-independent manner and with size selectivity. Here we demonstrate a new approach for rapidly determining the quantities of siRNA using fluorescence resonance energy transfer (FRET) between the Carnation Italian ringspot virus (CIRV) p19-CFP fusion protein and Cy3-labeled siRNA. The CIRV p19 fusion protein binds double-stranded siRNAs with nanomolar affinity as determined by FRET." @default.
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- W1979183998 date "2009-11-01" @default.
- W1979183998 modified "2023-09-27" @default.
- W1979183998 title "Erratum to “Studies of a viral suppressor of RNA silencing p19-CFP fusion protein: A FRET-based probe for sensing double-stranded fluorophore tagged small RNAs” [Biophysical Chemistry 143 (2009) 166–169]" @default.
- W1979183998 doi "https://doi.org/10.1016/j.bpc.2009.08.005" @default.
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