Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979238410> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1979238410 endingPage "243" @default.
- W1979238410 startingPage "235" @default.
- W1979238410 abstract "The glycerol dehydrogenase (GDH) from Bacillus stearothermophilus is inactivated by incubation with pyridoxal-5-phosphate (PALP). The complex formed between the two can be trapped by reduction with sodium borohydride to yield a protein with an absorbance band at 325 nm and a fluorescence emission band at 430 nm, typical of trapped pyridoxal-5-phosphate moieties. Total loss of catalytic activity of the enzyme is associated with the modification of approximately one equivalent of the reagent; the incorporation of the reagent and the loss of activity can be prevented by the additional presence of the oxidised or reduced coenzyme. Peptides derived from the labelled protein have been sequenced and have identified Lys-97 as the reactive residue. Site-directed mutagenesis had been used to replace Lys-97 by a His residue. This mutated enzyme has no catalytic activity and fluorescence spectroscopy studies suggest that it is unable to bind NADH." @default.
- W1979238410 created "2016-06-24" @default.
- W1979238410 creator A5010032895 @default.
- W1979238410 creator A5020078631 @default.
- W1979238410 creator A5058323629 @default.
- W1979238410 creator A5073183606 @default.
- W1979238410 creator A5083725647 @default.
- W1979238410 date "1993-10-01" @default.
- W1979238410 modified "2023-09-27" @default.
- W1979238410 title "The identification of a lysine residue reactive to pyridoxal-5-phosphate in the glycerol dehydrogenase from the thermophile Bacillus stearothermophilus" @default.
- W1979238410 cites W120446455 @default.
- W1979238410 cites W1538744914 @default.
- W1979238410 cites W1566161480 @default.
- W1979238410 cites W1577769874 @default.
- W1979238410 cites W1596205209 @default.
- W1979238410 cites W1630128553 @default.
- W1979238410 cites W173054628 @default.
- W1979238410 cites W1836475659 @default.
- W1979238410 cites W1916134635 @default.
- W1979238410 cites W1964393687 @default.
- W1979238410 cites W1964858907 @default.
- W1979238410 cites W1988233703 @default.
- W1979238410 cites W2018010694 @default.
- W1979238410 cites W2028622989 @default.
- W1979238410 cites W2038533609 @default.
- W1979238410 cites W2041887190 @default.
- W1979238410 cites W2043055897 @default.
- W1979238410 cites W2049105193 @default.
- W1979238410 cites W2055673460 @default.
- W1979238410 cites W2077180517 @default.
- W1979238410 cites W2336557764 @default.
- W1979238410 cites W2414981026 @default.
- W1979238410 cites W2888511473 @default.
- W1979238410 cites W4240038333 @default.
- W1979238410 cites W4256637238 @default.
- W1979238410 cites W61699629 @default.
- W1979238410 doi "https://doi.org/10.1016/0167-4838(93)90010-o" @default.
- W1979238410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8399385" @default.
- W1979238410 hasPublicationYear "1993" @default.
- W1979238410 type Work @default.
- W1979238410 sameAs 1979238410 @default.
- W1979238410 citedByCount "6" @default.
- W1979238410 countsByYear W19792384102015 @default.
- W1979238410 crossrefType "journal-article" @default.
- W1979238410 hasAuthorship W1979238410A5010032895 @default.
- W1979238410 hasAuthorship W1979238410A5020078631 @default.
- W1979238410 hasAuthorship W1979238410A5058323629 @default.
- W1979238410 hasAuthorship W1979238410A5073183606 @default.
- W1979238410 hasAuthorship W1979238410A5083725647 @default.
- W1979238410 hasConcept C178790620 @default.
- W1979238410 hasConcept C181199279 @default.
- W1979238410 hasConcept C185592680 @default.
- W1979238410 hasConcept C197957613 @default.
- W1979238410 hasConcept C2776016237 @default.
- W1979238410 hasConcept C2776317432 @default.
- W1979238410 hasConcept C2776834122 @default.
- W1979238410 hasConcept C2778428456 @default.
- W1979238410 hasConcept C2780859938 @default.
- W1979238410 hasConcept C2781338088 @default.
- W1979238410 hasConcept C27911776 @default.
- W1979238410 hasConcept C40875361 @default.
- W1979238410 hasConcept C515207424 @default.
- W1979238410 hasConcept C55493867 @default.
- W1979238410 hasConceptScore W1979238410C178790620 @default.
- W1979238410 hasConceptScore W1979238410C181199279 @default.
- W1979238410 hasConceptScore W1979238410C185592680 @default.
- W1979238410 hasConceptScore W1979238410C197957613 @default.
- W1979238410 hasConceptScore W1979238410C2776016237 @default.
- W1979238410 hasConceptScore W1979238410C2776317432 @default.
- W1979238410 hasConceptScore W1979238410C2776834122 @default.
- W1979238410 hasConceptScore W1979238410C2778428456 @default.
- W1979238410 hasConceptScore W1979238410C2780859938 @default.
- W1979238410 hasConceptScore W1979238410C2781338088 @default.
- W1979238410 hasConceptScore W1979238410C27911776 @default.
- W1979238410 hasConceptScore W1979238410C40875361 @default.
- W1979238410 hasConceptScore W1979238410C515207424 @default.
- W1979238410 hasConceptScore W1979238410C55493867 @default.
- W1979238410 hasIssue "2" @default.
- W1979238410 hasLocation W19792384101 @default.
- W1979238410 hasLocation W19792384102 @default.
- W1979238410 hasOpenAccess W1979238410 @default.
- W1979238410 hasPrimaryLocation W19792384101 @default.
- W1979238410 hasRelatedWork W1868493353 @default.
- W1979238410 hasRelatedWork W1971418776 @default.
- W1979238410 hasRelatedWork W2045902189 @default.
- W1979238410 hasRelatedWork W2066641096 @default.
- W1979238410 hasRelatedWork W2069185390 @default.
- W1979238410 hasRelatedWork W2071806336 @default.
- W1979238410 hasRelatedWork W2094248253 @default.
- W1979238410 hasRelatedWork W2110249947 @default.
- W1979238410 hasRelatedWork W2395085887 @default.
- W1979238410 hasRelatedWork W2811032335 @default.
- W1979238410 hasVolume "1202" @default.
- W1979238410 isParatext "false" @default.
- W1979238410 isRetracted "false" @default.
- W1979238410 magId "1979238410" @default.
- W1979238410 workType "article" @default.