FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979273883> ?p ?o ?g. }

• W1979273883 endingPage "10845" @default.
• W1979273883 startingPage "10839" @default.
• W1979273883 abstract "Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is analyzed, and from that, the molecular weights of free and bound molecules can be calculated. We have applied FCS to study the receptor diversity for the neuropeptide galanin (GAL) in cultured cells. FCS measurement of the fluorophore rhodamine-labeled GAL (Rh-GAL) has been performed in 0.2-fL confocal volume elements of the laser beam. The analysis of autocorrelation functions of Rh-GAL in solution above cells and at cell membranes demonstrates that the diffusion time of unbound Rh-GAL is 0.16 ms, whereas diffusion times of membrane-bound Rh-GAL are 22 and 700 ms. Because both of the diffusion times (22 and 700 ms) are much longer as compared to that of unbound Rh-GAL, they correspond to slow-diffusing complexes when Rh-GAL is bound to the cell membranes. Addition of excess nonlabeled GAL is accompanied by competitive displacement. Full saturation of the GAL binding is obtained at nanomolar concentrations. Scatchard analysis of binding data reveal one binding process, assuming one binding site per Rh-GAL (n = 1). On the other hand, the appearance of two diffusion times, 22 and 700 ms, suggests the existence of two subpopulations of GAL receptor complexes or two subtypes of GAL receptor not detected before. This makes an important point that FCS permits the identification of receptors, which were not possible to detect before by conventional binding techniques. The inhibitory effect of pertussis toxin on the GAL binding considers a G-protein-involved allosteric system, important for the clarification of essential steps in the G-protein-related signal transduction. This study is of pharmaceutical significance, since it will provide insights into how FCS can be used as a rapid technique for studying ligand-receptor interactions in living cells, which is one step forward for large-scale drug screening in cell cultures." @default.
• W1979273883 created "2016-06-24" @default.
• W1979273883 creator A5029171317 @default.
• W1979273883 creator A5045155025 @default.
• W1979273883 creator A5056284790 @default.
• W1979273883 creator A5057501316 @default.
• W1979273883 creator A5083582629 @default.
• W1979273883 date "2001-08-15" @default.
• W1979273883 modified "2023-10-18" @default.
• W1979273883 title "Fluorescence Correlation Spectroscopy Detects Galanin Receptor Diversity on Insulinoma Cells" @default.
• W1979273883 cites W1963500191 @default.
• W1979273883 cites W1969073916 @default.
• W1979273883 cites W1981765336 @default.
• W1979273883 cites W1994028876 @default.
• W1979273883 cites W1995829602 @default.
• W1979273883 cites W1998093640 @default.
• W1979273883 cites W2002247711 @default.
• W1979273883 cites W2059085488 @default.
• W1979273883 cites W2064136961 @default.
• W1979273883 cites W2065586627 @default.
• W1979273883 cites W2085245114 @default.
• W1979273883 cites W2087070363 @default.
• W1979273883 cites W2103506045 @default.
• W1979273883 cites W2341129282 @default.
• W1979273883 doi "https://doi.org/10.1021/bi010514q" @default.
• W1979273883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11535060" @default.
• W1979273883 hasPublicationYear "2001" @default.
• W1979273883 type Work @default.
• W1979273883 sameAs 1979273883 @default.
• W1979273883 citedByCount "41" @default.
• W1979273883 countsByYear W19792738832012 @default.
• W1979273883 countsByYear W19792738832013 @default.
• W1979273883 countsByYear W19792738832014 @default.
• W1979273883 countsByYear W19792738832015 @default.
• W1979273883 countsByYear W19792738832016 @default.
• W1979273883 countsByYear W19792738832018 @default.
• W1979273883 crossrefType "journal-article" @default.
• W1979273883 hasAuthorship W1979273883A5029171317 @default.
• W1979273883 hasAuthorship W1979273883A5045155025 @default.
• W1979273883 hasAuthorship W1979273883A5056284790 @default.
• W1979273883 hasAuthorship W1979273883A5057501316 @default.
• W1979273883 hasAuthorship W1979273883A5083582629 @default.
• W1979273883 hasConcept C113196181 @default.
• W1979273883 hasConcept C118303440 @default.
• W1979273883 hasConcept C121332964 @default.
• W1979273883 hasConcept C12554922 @default.
• W1979273883 hasConcept C144618238 @default.
• W1979273883 hasConcept C170493617 @default.
• W1979273883 hasConcept C178790620 @default.
• W1979273883 hasConcept C185592680 @default.
• W1979273883 hasConcept C19519825 @default.
• W1979273883 hasConcept C32909587 @default.
• W1979273883 hasConcept C41625074 @default.
• W1979273883 hasConcept C43617362 @default.
• W1979273883 hasConcept C55493867 @default.
• W1979273883 hasConcept C62520636 @default.
• W1979273883 hasConcept C86803240 @default.
• W1979273883 hasConcept C91881484 @default.
• W1979273883 hasConceptScore W1979273883C113196181 @default.
• W1979273883 hasConceptScore W1979273883C118303440 @default.
• W1979273883 hasConceptScore W1979273883C121332964 @default.
• W1979273883 hasConceptScore W1979273883C12554922 @default.
• W1979273883 hasConceptScore W1979273883C144618238 @default.
• W1979273883 hasConceptScore W1979273883C170493617 @default.
• W1979273883 hasConceptScore W1979273883C178790620 @default.
• W1979273883 hasConceptScore W1979273883C185592680 @default.
• W1979273883 hasConceptScore W1979273883C19519825 @default.
• W1979273883 hasConceptScore W1979273883C32909587 @default.
• W1979273883 hasConceptScore W1979273883C41625074 @default.
• W1979273883 hasConceptScore W1979273883C43617362 @default.
• W1979273883 hasConceptScore W1979273883C55493867 @default.
• W1979273883 hasConceptScore W1979273883C62520636 @default.
• W1979273883 hasConceptScore W1979273883C86803240 @default.
• W1979273883 hasConceptScore W1979273883C91881484 @default.
• W1979273883 hasIssue "36" @default.
• W1979273883 hasLocation W19792738831 @default.
• W1979273883 hasLocation W19792738832 @default.
• W1979273883 hasOpenAccess W1979273883 @default.
• W1979273883 hasPrimaryLocation W19792738831 @default.
• W1979273883 hasRelatedWork W1514500309 @default.
• W1979273883 hasRelatedWork W1671774171 @default.
• W1979273883 hasRelatedWork W1965040939 @default.
• W1979273883 hasRelatedWork W1990737682 @default.
• W1979273883 hasRelatedWork W1991356650 @default.
• W1979273883 hasRelatedWork W1999324844 @default.
• W1979273883 hasRelatedWork W2128735823 @default.
• W1979273883 hasRelatedWork W2163005939 @default.
• W1979273883 hasRelatedWork W2986446519 @default.
• W1979273883 hasRelatedWork W4285164382 @default.
• W1979273883 hasVolume "40" @default.
• W1979273883 isParatext "false" @default.
• W1979273883 isRetracted "false" @default.
• W1979273883 magId "1979273883" @default.
• W1979273883 workType "article" @default.