Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979286502> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1979286502 endingPage "9074" @default.
- W1979286502 startingPage "9061" @default.
- W1979286502 abstract "The Na,K- and H,K-ATPases are plasma membrane enzymes responsible for the active exchange of extracellular K+ for cytoplasmic Na+ or H+, respectively. At present, the structural determinants for the specific function of these ATPases remain poorly understood. To investigate the cation selectivity of these ATPases, we constructed a series of Na,K-ATPase mutants in which residues in the membrane spanning segments of the α subunit were changed to the corresponding residues common to gastric H,K-ATPases. Thus, mutants were created with substitutions in transmembrane domains TM1, TM4, TM5, TM6, TM7, and TM8 independently or together (designated TMAll). The function of each mutant was assessed after coexpression with the β subunit in Sf-9 cells using baculoviruses. The enzymatic properties of TM1, TM7, and TM8 mutants were similar to the wild-type Na,K-ATPase, and while TM5 showed modest changes in apparent affinity for Na+, TM4, TM6, and TMAll displayed an abnormal activity. This resulted in a Na+-independent hydrolysis of ATP, a 2-fold higher K0.5 for Na+ activation, and the ability to function at low pH. These results suggest a loss of discrimination for Na+ over H+ for the enzymes. In addition, TM4, TM6, and TMAll mutants exhibited a 1.5-fold lower affinity for K+ and a 4−5-fold decreased sensitivity to vanadate. Altogether, these results provide evidence that residues in transmembrane domains 4 and 6 of the α subunit of the Na,K-ATPase play an important role in determining the specific cation selectivity of the enzyme and also its E1/E2 conformational equilibrium." @default.
- W1979286502 created "2016-06-24" @default.
- W1979286502 creator A5037666188 @default.
- W1979286502 creator A5058801534 @default.
- W1979286502 date "2004-06-19" @default.
- W1979286502 modified "2023-09-27" @default.
- W1979286502 title "Residues within Transmembrane Domains 4 and 6 of the Na,K-ATPase α Subunit Are Important for Na+ Selectivity" @default.
- W1979286502 cites W1548022389 @default.
- W1979286502 cites W1550258731 @default.
- W1979286502 cites W1562290896 @default.
- W1979286502 cites W1572051922 @default.
- W1979286502 cites W1604272784 @default.
- W1979286502 cites W1608843362 @default.
- W1979286502 cites W1965899958 @default.
- W1979286502 cites W1972904714 @default.
- W1979286502 cites W1978339941 @default.
- W1979286502 cites W1982206979 @default.
- W1979286502 cites W2000247378 @default.
- W1979286502 cites W2015493792 @default.
- W1979286502 cites W2031320408 @default.
- W1979286502 cites W2047801965 @default.
- W1979286502 cites W2057522428 @default.
- W1979286502 cites W2078814797 @default.
- W1979286502 cites W2081226748 @default.
- W1979286502 cites W2110076427 @default.
- W1979286502 cites W2131271627 @default.
- W1979286502 cites W2142048790 @default.
- W1979286502 doi "https://doi.org/10.1021/bi049484s" @default.
- W1979286502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15248763" @default.
- W1979286502 hasPublicationYear "2004" @default.
- W1979286502 type Work @default.
- W1979286502 sameAs 1979286502 @default.
- W1979286502 citedByCount "6" @default.
- W1979286502 countsByYear W19792865022013 @default.
- W1979286502 countsByYear W19792865022014 @default.
- W1979286502 crossrefType "journal-article" @default.
- W1979286502 hasAuthorship W1979286502A5037666188 @default.
- W1979286502 hasAuthorship W1979286502A5058801534 @default.
- W1979286502 hasConcept C104292427 @default.
- W1979286502 hasConcept C104317684 @default.
- W1979286502 hasConcept C118792377 @default.
- W1979286502 hasConcept C118892022 @default.
- W1979286502 hasConcept C12554922 @default.
- W1979286502 hasConcept C141315368 @default.
- W1979286502 hasConcept C143065580 @default.
- W1979286502 hasConcept C161790260 @default.
- W1979286502 hasConcept C170493617 @default.
- W1979286502 hasConcept C181199279 @default.
- W1979286502 hasConcept C185592680 @default.
- W1979286502 hasConcept C23265538 @default.
- W1979286502 hasConcept C24530287 @default.
- W1979286502 hasConcept C2781116636 @default.
- W1979286502 hasConcept C28406088 @default.
- W1979286502 hasConcept C41625074 @default.
- W1979286502 hasConcept C55493867 @default.
- W1979286502 hasConcept C86803240 @default.
- W1979286502 hasConceptScore W1979286502C104292427 @default.
- W1979286502 hasConceptScore W1979286502C104317684 @default.
- W1979286502 hasConceptScore W1979286502C118792377 @default.
- W1979286502 hasConceptScore W1979286502C118892022 @default.
- W1979286502 hasConceptScore W1979286502C12554922 @default.
- W1979286502 hasConceptScore W1979286502C141315368 @default.
- W1979286502 hasConceptScore W1979286502C143065580 @default.
- W1979286502 hasConceptScore W1979286502C161790260 @default.
- W1979286502 hasConceptScore W1979286502C170493617 @default.
- W1979286502 hasConceptScore W1979286502C181199279 @default.
- W1979286502 hasConceptScore W1979286502C185592680 @default.
- W1979286502 hasConceptScore W1979286502C23265538 @default.
- W1979286502 hasConceptScore W1979286502C24530287 @default.
- W1979286502 hasConceptScore W1979286502C2781116636 @default.
- W1979286502 hasConceptScore W1979286502C28406088 @default.
- W1979286502 hasConceptScore W1979286502C41625074 @default.
- W1979286502 hasConceptScore W1979286502C55493867 @default.
- W1979286502 hasConceptScore W1979286502C86803240 @default.
- W1979286502 hasIssue "28" @default.
- W1979286502 hasLocation W19792865021 @default.
- W1979286502 hasLocation W19792865022 @default.
- W1979286502 hasOpenAccess W1979286502 @default.
- W1979286502 hasPrimaryLocation W19792865021 @default.
- W1979286502 hasRelatedWork W1538027735 @default.
- W1979286502 hasRelatedWork W1973495335 @default.
- W1979286502 hasRelatedWork W1979286502 @default.
- W1979286502 hasRelatedWork W2050700773 @default.
- W1979286502 hasRelatedWork W2059655686 @default.
- W1979286502 hasRelatedWork W2121017866 @default.
- W1979286502 hasRelatedWork W2462623526 @default.
- W1979286502 hasRelatedWork W2561134176 @default.
- W1979286502 hasRelatedWork W2809277775 @default.
- W1979286502 hasRelatedWork W4293462144 @default.
- W1979286502 hasVolume "43" @default.
- W1979286502 isParatext "false" @default.
- W1979286502 isRetracted "false" @default.
- W1979286502 magId "1979286502" @default.
- W1979286502 workType "article" @default.