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- W1979303661 abstract "A procedure is described for assessing the proportion of lymphocyte populations which transform in vitro under the influence of phyto-hæmagglutinin (P.H.A.). Mechanical dispersal of white-blood-cell clumps in P.H.A. cultures permits accurate cell-counting, allowing corrections to be made of the values given by the differential counts for the presence of daughter cells. Losses are allowed for in the assessment of proliferation by counting the cells remaining in matching cultures in which proliferation has been prevented by the presence of colchicine. Application of this procedure to blood-lymphocyte populations from 8 healthy people on a morphological basis of assessment of transformation gave values in the range 45-73% (average, 60%) for 3-day cultures. The relation of these values to the proportions capable of transformation depends partly on the extent of lymphocyte death. The need is emphasised for carbon dioxide in the air-phase of the cultures to prevent lymphocyte death." @default.
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- W1979303661 date "1968-11-01" @default.
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- W1979303661 title "DEATH AND DIVISION OF LYMPHOCYTES" @default.
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- W1979303661 doi "https://doi.org/10.1016/s0140-6736(68)91583-3" @default.
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