FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979313552> ?p ?o ?g. }

• W1979313552 endingPage "153" @default.
• W1979313552 startingPage "143" @default.
• W1979313552 abstract "Abstract The objectives of this study were to develop a serum‐free system for culturing canine oral keratinocytes, the construction and characterization of a canine ex vivo produced oral mucosa equivalent (EVPOME), and transduction green fluorescent protein (GFP) into keratinocytes as a post‐grafting tracking marker. Dissociated canine buccal mucosa keratinocytes were cultured in a chemically defined serum‐free medium, Epilife™. First‐passage keratinocytes were transfected with the GFP gene using a lentiviral vector, sorted by flow cytometer and seeded onto a dermal equivalent, AlloDerm® to form EVPOMEs. The EVPOME was characterized by histology and immunohistochemistry, for p63, Ki‐67, and involucrin. Laser confocal microscopy was used to locate GFP‐transfected keratinocytes within the EVPOME. Cultured canine oral keratinocytes grew rapidly over the first three passages and then the proliferative rate decreased. The canine EVPOME formed a well‐stratified epithelial layer. The majority of p63 and Ki‐67 immunopositive cells were located in the basal layer whereas cytoplasmic involucrin expression was seen in the suprabasal layers, similar to native canine buccal mucosa. Under laser confocal microscopy, significant green fluorescence was observed throughout the EVPOME. In conclusion, canine EVPOMEs were successfully fabricated in a defined serum‐free system with similar characteristics to native buccal mucosa. GFP‐transfected canine oral keratinocytes could be identified within the EVPOME. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 143–153, 2004" @default.
• W1979313552 created "2016-06-24" @default.
• W1979313552 creator A5005919966 @default.
• W1979313552 creator A5037061802 @default.
• W1979313552 creator A5066838365 @default.
• W1979313552 creator A5083443593 @default.
• W1979313552 date "2004-08-18" @default.
• W1979313552 modified "2023-10-17" @default.
• W1979313552 title "Development and characterization of a canine oral mucosa equivalent in a serum-free environment" @default.
• W1979313552 cites W1978732072 @default.
• W1979313552 cites W1981899137 @default.
• W1979313552 cites W1991635026 @default.
• W1979313552 cites W2001652055 @default.
• W1979313552 cites W2014938226 @default.
• W1979313552 cites W2020653282 @default.
• W1979313552 cites W2021815726 @default.
• W1979313552 cites W2025497355 @default.
• W1979313552 cites W2027380981 @default.
• W1979313552 cites W2028408635 @default.
• W1979313552 cites W2036385637 @default.
• W1979313552 cites W2050682938 @default.
• W1979313552 cites W2052874341 @default.
• W1979313552 cites W2055171571 @default.
• W1979313552 cites W2059881996 @default.
• W1979313552 cites W2071308813 @default.
• W1979313552 cites W2071831534 @default.
• W1979313552 cites W2084111825 @default.
• W1979313552 cites W2089579355 @default.
• W1979313552 cites W2115311615 @default.
• W1979313552 cites W2125160088 @default.
• W1979313552 cites W2125787407 @default.
• W1979313552 cites W2162749230 @default.
• W1979313552 cites W2168211018 @default.
• W1979313552 cites W2169277223 @default.
• W1979313552 cites W2169646754 @default.
• W1979313552 cites W2171272444 @default.
• W1979313552 cites W2171918816 @default.
• W1979313552 cites W2171951564 @default.
• W1979313552 cites W2331430202 @default.
• W1979313552 cites W4244010968 @default.
• W1979313552 cites W4244379414 @default.
• W1979313552 cites W1981117456 @default.
• W1979313552 doi "https://doi.org/10.1002/jbm.a.30144" @default.
• W1979313552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15368264" @default.
• W1979313552 hasPublicationYear "2004" @default.
• W1979313552 type Work @default.
• W1979313552 sameAs 1979313552 @default.
• W1979313552 citedByCount "21" @default.
• W1979313552 countsByYear W19793135522012 @default.
• W1979313552 countsByYear W19793135522013 @default.
• W1979313552 countsByYear W19793135522014 @default.
• W1979313552 countsByYear W19793135522015 @default.
• W1979313552 countsByYear W19793135522016 @default.
• W1979313552 countsByYear W19793135522017 @default.
• W1979313552 countsByYear W19793135522019 @default.
• W1979313552 countsByYear W19793135522020 @default.
• W1979313552 countsByYear W19793135522021 @default.
• W1979313552 crossrefType "journal-article" @default.
• W1979313552 hasAuthorship W1979313552A5005919966 @default.
• W1979313552 hasAuthorship W1979313552A5037061802 @default.
• W1979313552 hasAuthorship W1979313552A5066838365 @default.
• W1979313552 hasAuthorship W1979313552A5083443593 @default.
• W1979313552 hasBestOaLocation W19793135522 @default.
• W1979313552 hasConcept C104317684 @default.
• W1979313552 hasConcept C142613039 @default.
• W1979313552 hasConcept C142724271 @default.
• W1979313552 hasConcept C150903083 @default.
• W1979313552 hasConcept C153911025 @default.
• W1979313552 hasConcept C202751555 @default.
• W1979313552 hasConcept C207001950 @default.
• W1979313552 hasConcept C26291073 @default.
• W1979313552 hasConcept C2777074287 @default.
• W1979313552 hasConcept C2777577635 @default.
• W1979313552 hasConcept C2779178360 @default.
• W1979313552 hasConcept C2779409057 @default.
• W1979313552 hasConcept C29694066 @default.
• W1979313552 hasConcept C2986629439 @default.
• W1979313552 hasConcept C2991923528 @default.
• W1979313552 hasConcept C54009773 @default.
• W1979313552 hasConcept C54355233 @default.
• W1979313552 hasConcept C55493867 @default.
• W1979313552 hasConcept C71924100 @default.
• W1979313552 hasConcept C81885089 @default.
• W1979313552 hasConcept C86803240 @default.
• W1979313552 hasConcept C95444343 @default.
• W1979313552 hasConceptScore W1979313552C104317684 @default.
• W1979313552 hasConceptScore W1979313552C142613039 @default.
• W1979313552 hasConceptScore W1979313552C142724271 @default.
• W1979313552 hasConceptScore W1979313552C150903083 @default.
• W1979313552 hasConceptScore W1979313552C153911025 @default.
• W1979313552 hasConceptScore W1979313552C202751555 @default.
• W1979313552 hasConceptScore W1979313552C207001950 @default.
• W1979313552 hasConceptScore W1979313552C26291073 @default.
• W1979313552 hasConceptScore W1979313552C2777074287 @default.
• W1979313552 hasConceptScore W1979313552C2777577635 @default.
• W1979313552 hasConceptScore W1979313552C2779178360 @default.
• W1979313552 hasConceptScore W1979313552C2779409057 @default.
• W1979313552 hasConceptScore W1979313552C29694066 @default.

• next