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- W1979319564 abstract "Golgi α1,2-mannosidase I is involved in the N-linked oligosaccharide processing pathway. In this study, two truncated genes encoding for human Golgi α1,2-mannosidase I (hManIA2: amino acids 127–626 and hManIC: amino acids 118–617) were expressed in Escherichia coli to characterize the enzymes. These genes were fused to a T7 protein tag and a histidine tag at the N- and C-terminal ends, respectively, and purified using Co2+ affinity chromatography. The properties including optimal temperature, optimal pH, and substrate specificity of the purified enzymes were investigated by HPLC using pyridylamino (PA)-labeled oligosaccharides as substrates. The stability of hManIA2 was dependent on the presence of Ca2+, which was also required for its activity. On the other hand, hManIC was stable in the absence of Ca2+, even though Ca2+ was also effective for the activity of hManIC. While the similarity of the amino acid sequences is over 60%, hManIA2 and hManIC showed different substrate specificities particularly toward M9A and M8C." @default.
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- W1979319564 date "2011-07-01" @default.
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- W1979319564 title "Improved expression and characterization of recombinant human Golgi α1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli" @default.
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- W1979319564 doi "https://doi.org/10.1016/j.jbiosc.2011.02.013" @default.
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