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- W1979387350 abstract "Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg mL(-1) (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg mL(-1) (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg mL(-1) (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation <20%). Quantification curves for the ten toxins were also evaluated in clinical samples (serum, plasma, nasal fluid, saliva, stool, and urine) and environmental samples (apple juice, milk and baby food) with overall minimal matrix effects. The multiplex assays were highly specific, with little cross-reactivity observed between the selected toxin antibodies. The results demonstrate a multiplex microarray that improves current immunoassay sensitivity for biological warfare agents in buffer, clinical, and environmental samples." @default.
- W1979387350 created "2016-06-24" @default.
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- W1979387350 date "2014-01-01" @default.
- W1979387350 modified "2023-10-12" @default.
- W1979387350 title "Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins" @default.
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- W1979387350 cites W1547803736 @default.
- W1979387350 cites W1556008477 @default.
- W1979387350 cites W1947863176 @default.
- W1979387350 cites W1964435848 @default.
- W1979387350 cites W1964783811 @default.
- W1979387350 cites W1967073147 @default.
- W1979387350 cites W1968725817 @default.
- W1979387350 cites W1971765133 @default.
- W1979387350 cites W1971799980 @default.
- W1979387350 cites W1972447472 @default.
- W1979387350 cites W1978197740 @default.
- W1979387350 cites W1978276153 @default.
- W1979387350 cites W1979728732 @default.
- W1979387350 cites W1996626616 @default.
- W1979387350 cites W2000674580 @default.
- W1979387350 cites W2005895326 @default.
- W1979387350 cites W2008137659 @default.
- W1979387350 cites W2011603383 @default.
- W1979387350 cites W2013682889 @default.
- W1979387350 cites W2013757167 @default.
- W1979387350 cites W2017267009 @default.
- W1979387350 cites W2017880027 @default.
- W1979387350 cites W2019590054 @default.
- W1979387350 cites W2021258548 @default.
- W1979387350 cites W2023645250 @default.
- W1979387350 cites W2027306447 @default.
- W1979387350 cites W2028780872 @default.
- W1979387350 cites W2030504385 @default.
- W1979387350 cites W2039820935 @default.
- W1979387350 cites W2042960267 @default.
- W1979387350 cites W2047365118 @default.
- W1979387350 cites W204869595 @default.
- W1979387350 cites W2053694497 @default.
- W1979387350 cites W2063208338 @default.
- W1979387350 cites W2066352483 @default.
- W1979387350 cites W2069635959 @default.
- W1979387350 cites W2071743575 @default.
- W1979387350 cites W2072413451 @default.
- W1979387350 cites W2075825292 @default.
- W1979387350 cites W2080428561 @default.
- W1979387350 cites W2082734948 @default.
- W1979387350 cites W2088808595 @default.
- W1979387350 cites W2090162339 @default.
- W1979387350 cites W2090517365 @default.
- W1979387350 cites W2090739893 @default.
- W1979387350 cites W2091822825 @default.
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- W1979387350 cites W2102029603 @default.
- W1979387350 cites W2104237723 @default.
- W1979387350 cites W2108780469 @default.
- W1979387350 cites W2116555954 @default.
- W1979387350 cites W2116973295 @default.
- W1979387350 cites W2118154806 @default.
- W1979387350 cites W2123805379 @default.
- W1979387350 cites W2133511513 @default.
- W1979387350 cites W2137075021 @default.
- W1979387350 cites W2137889830 @default.
- W1979387350 cites W2144951924 @default.
- W1979387350 cites W2156679520 @default.
- W1979387350 cites W2158474857 @default.
- W1979387350 cites W2161165456 @default.
- W1979387350 cites W2169606215 @default.
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- W1979387350 cites W2170899493 @default.
- W1979387350 cites W2319320711 @default.
- W1979387350 cites W2321671266 @default.
- W1979387350 cites W2329159859 @default.
- W1979387350 cites W4211083870 @default.
- W1979387350 cites W4241019103 @default.
- W1979387350 cites W4243170482 @default.
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- W1979387350 doi "https://doi.org/10.1039/c4an01270d" @default.
- W1979387350 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6540756" @default.
- W1979387350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25112421" @default.
- W1979387350 hasPublicationYear "2014" @default.
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