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- W1979387418 abstract "Labeling cells and tissues with fluorescent probes such as organic dyes and quantum dots (QDots) is a widespread and successful technique for studying molecular dynamics both in vitro and in vivo. However, those probes usually suffer from photophysical/photochemical processes, such us blinking and photobleaching, limiting their utilization. The main challenges in probe design are to improve single molecule fluorescence detection, and to provide higher stability against photobleaching. In the last few years, metallic nanoparticles (NPs) of various sizes, shapes, and materials have been used as new labels for cellular microscopy. This is in part because, unlike common organic dyes and QDots, metallic NPs do not bleach or blink upon continuous illumination, are extremely stable, very bright and their luminescence spans over the visible spectrum. Their excellent optical and chemical properties make them an attractive contrast agent for cell imaging both in vitro and in vivo. In this work we show some of their unique properties, and examples that illustrate their capabilities when used as probes in a single particle tracking experiment. This work was supported by the National Institutes of Health grant number P41-RR003155 and the University of California, Irvine." @default.
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- W1979387418 date "2012-01-01" @default.
- W1979387418 modified "2023-10-16" @default.
- W1979387418 title "Using Metallic Nanoparticles for Nanometer-Resolution Optical Imaging in Living Cells: Photophysical Properties and Applications" @default.
- W1979387418 doi "https://doi.org/10.1016/j.bpj.2011.11.1143" @default.
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