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- W1979387706 abstract "A bioelectrochemical in vivo flow-injection system with an on-line microdialysis sampling is proposed for the assay of the trace amounts of L-glutamate released from rat brain cells. The system includes a microdialysis probe, two immobilized enzyme reactors, a six-way autoinjector, and a poly(1,2-diaminobenzene) film-coated platinum electrode. In the first stage of the operation, the dialysate from the probe was delivered to the sample loop of the six-way autoinjector by perfusing Ringer's solution for 3 min at 2 μL min−1. The calibration of the detection system including enzyme reactors was performed simultaneously. In the second stage, the dialysate in sample loop was automatically injected for 30 s into the flow-injection line and assayed. An L-glutamate oxidase/glutamate dehydrogenase coimmobilized reactor was used as an on-line amplifier based on the substrate recycling. To improve the substrate specificity of this reactor, acidic pretreatment and 6-diazo-5-oxo-L-norleucine treatment were done. The resulting enzyme reactor worked as a specific reactor of L-glutamate␣involving amplification (amplification factor was about 60 at a carrier flow-rate of 100 μL min−1). The cycle was also initiated with 2-oxoglutarate, and so saccharopine dehydrogenase reactor was positioned in series before the amplifier reactor to remove 2-oxoglutarate. By the present method, the variations of L-glutamate level released from rat brain cells were assayed in vivo and also after continuous stimulation of KCl, to demonstrate the reliability of the system. Analytical speed was 20 dialysates h−1 and the detection limit was less than 0.5 μM." @default.
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- W1979387706 date "2001-11-01" @default.
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- W1979387706 title "An Electrochemical In Vivo Flow-Injection System for Highly Selective and Sensitive Detection ofL-Glutamate Using Enzyme Reactor Involving Amplification" @default.
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- W1979387706 doi "https://doi.org/10.1002/1521-4109(200111)13:16<1361::aid-elan1361>3.0.co;2-d" @default.
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