FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979450156> ?p ?o ?g. }

• W1979450156 endingPage "1103" @default.
• W1979450156 startingPage "1095" @default.
• W1979450156 abstract "ObjectiveTo determine whether OE-E6/E7, an immortalized human oviductal epithelial cell line, expresses oviductin messenger RNA (mRNA) and its translated protein.DesignTransmission electron microscopy was employed to characterize the morphology of OE-E6/E7 cells followed by reverse-transcription polymerase chain reaction (PCR) analysis of oviductin mRNA and sequencing of the nested-PCR product. Confocal microscopy was used, using a polyclonal antibody against human oviductin and Con A as a marker for mannose residues, to reveal the colocalization of human oviduct-specific glycoprotein with the endoplasmic reticulum and Golgi compartments.SettingUniversity-based anatomy and cell biology department.Patient(s)Women undergoing laparoscopy for tubal ligation or hysterectomy due to uterine fibroma.Intervention(s)An immortalized OE-E6/E7 cell line was previously established using human oviductal epithelial cells. Electron microscopy, RT-PCR, sequencing, immunohistochemistry and confocal microscopy were performed.Main Outcome Measure(s)The presence of human oviductin mRNA and protein in OE-E6/E7 cells.Result(s)OE-E6/E7 cells retain morphological features characteristic of secretory cells and express human oviductin mRNA and its translated protein.Conclusion(s)OE-E6/E7 cells were characterized for the first time by electron microscopy and shown to exhibit histological features typical of secretory cells. Reverse-transcription PCR with sequencing and confocal microscopy showed, respectively, that human oviductin mRNA and protein are expressed in OE-E6/E7 cells. Our results suggest that OE-E6/E7 could be a useful tool for future studies of the function of human oviductin. To determine whether OE-E6/E7, an immortalized human oviductal epithelial cell line, expresses oviductin messenger RNA (mRNA) and its translated protein. Transmission electron microscopy was employed to characterize the morphology of OE-E6/E7 cells followed by reverse-transcription polymerase chain reaction (PCR) analysis of oviductin mRNA and sequencing of the nested-PCR product. Confocal microscopy was used, using a polyclonal antibody against human oviductin and Con A as a marker for mannose residues, to reveal the colocalization of human oviduct-specific glycoprotein with the endoplasmic reticulum and Golgi compartments. University-based anatomy and cell biology department. Women undergoing laparoscopy for tubal ligation or hysterectomy due to uterine fibroma. An immortalized OE-E6/E7 cell line was previously established using human oviductal epithelial cells. Electron microscopy, RT-PCR, sequencing, immunohistochemistry and confocal microscopy were performed. The presence of human oviductin mRNA and protein in OE-E6/E7 cells. OE-E6/E7 cells retain morphological features characteristic of secretory cells and express human oviductin mRNA and its translated protein. OE-E6/E7 cells were characterized for the first time by electron microscopy and shown to exhibit histological features typical of secretory cells. Reverse-transcription PCR with sequencing and confocal microscopy showed, respectively, that human oviductin mRNA and protein are expressed in OE-E6/E7 cells. Our results suggest that OE-E6/E7 could be a useful tool for future studies of the function of human oviductin." @default.
• W1979450156 created "2016-06-24" @default.
• W1979450156 creator A5012578991 @default.
• W1979450156 creator A5029067036 @default.
• W1979450156 creator A5037650050 @default.
• W1979450156 creator A5045092437 @default.
• W1979450156 creator A5049670177 @default.
• W1979450156 creator A5069024030 @default.
• W1979450156 date "2005-10-01" @default.
• W1979450156 modified "2023-10-10" @default.
• W1979450156 title "Expression of human oviductin in an immortalized human oviductal cell line" @default.
• W1979450156 cites W1945320738 @default.
• W1979450156 cites W1976002046 @default.
• W1979450156 cites W1988641397 @default.
• W1979450156 cites W1989739763 @default.
• W1979450156 cites W1997713153 @default.
• W1979450156 cites W2009050439 @default.
• W1979450156 cites W2019661716 @default.
• W1979450156 cites W2021506736 @default.
• W1979450156 cites W2028153286 @default.
• W1979450156 cites W2040311391 @default.
• W1979450156 cites W2041782023 @default.
• W1979450156 cites W2043405195 @default.
• W1979450156 cites W2056525767 @default.
• W1979450156 cites W2075917891 @default.
• W1979450156 cites W2077873676 @default.
• W1979450156 cites W2078548507 @default.
• W1979450156 cites W2081246910 @default.
• W1979450156 cites W2088577272 @default.
• W1979450156 cites W2094509587 @default.
• W1979450156 cites W2100245273 @default.
• W1979450156 cites W2108376889 @default.
• W1979450156 cites W2111886394 @default.
• W1979450156 cites W2126910954 @default.
• W1979450156 cites W2128877425 @default.
• W1979450156 cites W2130114996 @default.
• W1979450156 cites W2150852055 @default.
• W1979450156 cites W2154101363 @default.
• W1979450156 cites W2159600332 @default.
• W1979450156 cites W2163567889 @default.
• W1979450156 cites W2163800008 @default.
• W1979450156 cites W2418971026 @default.
• W1979450156 doi "https://doi.org/10.1016/j.fertnstert.2005.06.006" @default.
• W1979450156 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16209999" @default.
• W1979450156 hasPublicationYear "2005" @default.
• W1979450156 type Work @default.
• W1979450156 sameAs 1979450156 @default.
• W1979450156 citedByCount "14" @default.
• W1979450156 countsByYear W19794501562012 @default.
• W1979450156 countsByYear W19794501562013 @default.
• W1979450156 countsByYear W19794501562014 @default.
• W1979450156 countsByYear W19794501562015 @default.
• W1979450156 countsByYear W19794501562016 @default.
• W1979450156 countsByYear W19794501562020 @default.
• W1979450156 crossrefType "journal-article" @default.
• W1979450156 hasAuthorship W1979450156A5012578991 @default.
• W1979450156 hasAuthorship W1979450156A5029067036 @default.
• W1979450156 hasAuthorship W1979450156A5037650050 @default.
• W1979450156 hasAuthorship W1979450156A5045092437 @default.
• W1979450156 hasAuthorship W1979450156A5049670177 @default.
• W1979450156 hasAuthorship W1979450156A5069024030 @default.
• W1979450156 hasBestOaLocation W19794501561 @default.
• W1979450156 hasConcept C104317684 @default.
• W1979450156 hasConcept C105580179 @default.
• W1979450156 hasConcept C153911025 @default.
• W1979450156 hasConcept C158617107 @default.
• W1979450156 hasConcept C203014093 @default.
• W1979450156 hasConcept C204232928 @default.
• W1979450156 hasConcept C2993140218 @default.
• W1979450156 hasConcept C54355233 @default.
• W1979450156 hasConcept C55493867 @default.
• W1979450156 hasConcept C6045943 @default.
• W1979450156 hasConcept C81885089 @default.
• W1979450156 hasConcept C86803240 @default.
• W1979450156 hasConcept C95444343 @default.
• W1979450156 hasConceptScore W1979450156C104317684 @default.
• W1979450156 hasConceptScore W1979450156C105580179 @default.
• W1979450156 hasConceptScore W1979450156C153911025 @default.
• W1979450156 hasConceptScore W1979450156C158617107 @default.
• W1979450156 hasConceptScore W1979450156C203014093 @default.
• W1979450156 hasConceptScore W1979450156C204232928 @default.
• W1979450156 hasConceptScore W1979450156C2993140218 @default.
• W1979450156 hasConceptScore W1979450156C54355233 @default.
• W1979450156 hasConceptScore W1979450156C55493867 @default.
• W1979450156 hasConceptScore W1979450156C6045943 @default.
• W1979450156 hasConceptScore W1979450156C81885089 @default.
• W1979450156 hasConceptScore W1979450156C86803240 @default.
• W1979450156 hasConceptScore W1979450156C95444343 @default.
• W1979450156 hasLocation W19794501561 @default.
• W1979450156 hasLocation W19794501562 @default.
• W1979450156 hasOpenAccess W1979450156 @default.
• W1979450156 hasPrimaryLocation W19794501561 @default.
• W1979450156 hasRelatedWork W1987109449 @default.
• W1979450156 hasRelatedWork W2056048169 @default.
• W1979450156 hasRelatedWork W2073604143 @default.
• W1979450156 hasRelatedWork W2083722908 @default.
• W1979450156 hasRelatedWork W2108744823 @default.
• W1979450156 hasRelatedWork W2167064994 @default.

• next