FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979467260> ?p ?o ?g. }

• W1979467260 endingPage "365" @default.
• W1979467260 startingPage "355" @default.
• W1979467260 abstract "Intra-molecular cross-linking has been suggested as a method of obtaining distance constraints that would help to develop structural models of proteins. Recent work published on intra-molecular cross-linking for protein structural studies has employed commercially available primary amine (lysine, the amino terminus) selective reagents. Previous work using these cross-linkers has shown that for several proteins of known structure, the number of cross-links that can be obtained experimentally may be small compared to what would be expected from the known structure, due to the relative reactivity, distribution and solvent accessibility of the lysines in the protein sequence. To overcome these limitations, we have investigated the use of cross-linking reagents that can react with other reactive side chains in proteins. We used 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E) and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO side chains can react to form “zero-length” cross-links with nearby primary amine containing residues, lysines (K) and the amino terminus (X), via the formation of a new amide bond. We also show that the EDC-activated DEO side chains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker arm of variable length. Using these reagents, we have found three new “zero-length” cross-links in ubiquitin consistent with its known structure (M1–E16, M1–E18 and K63–E64). Using the dihydrazide cross-linkers, we have identified two new cross-links (D21–D32 and E24–D32) unambiguously. Using a library of dihydrazide cross-linkers with varying arm length, we have shown that there is a minimum arm length required for the DEO–DEO cross-links of 5.8Å. These results show that additional structural information can be obtained by exploiting new cross-linker chemistry, increasing the probability that the protein target will yield sufficient distance constraints to develop a structural model." @default.
• W1979467260 created "2016-06-24" @default.
• W1979467260 creator A5003847961 @default.
• W1979467260 creator A5057356551 @default.
• W1979467260 date "2008-04-01" @default.
• W1979467260 modified "2023-10-01" @default.
• W1979467260 title "Intra-Molecular Cross-Linking of Acidic Residues for Protein Structure Studies" @default.
• W1979467260 cites W1463577123 @default.
• W1979467260 cites W1570847438 @default.
• W1979467260 cites W1653038558 @default.
• W1979467260 cites W1974621200 @default.
• W1979467260 cites W1974843618 @default.
• W1979467260 cites W1982522594 @default.
• W1979467260 cites W1995050941 @default.
• W1979467260 cites W2000590871 @default.
• W1979467260 cites W2012534919 @default.
• W1979467260 cites W2018272940 @default.
• W1979467260 cites W2029318495 @default.
• W1979467260 cites W2054664166 @default.
• W1979467260 cites W2065251660 @default.
• W1979467260 cites W2070247089 @default.
• W1979467260 cites W2123797085 @default.
• W1979467260 cites W2174983906 @default.
• W1979467260 doi "https://doi.org/10.1255/ejms.963" @default.
• W1979467260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19136724" @default.
• W1979467260 hasPublicationYear "2008" @default.
• W1979467260 type Work @default.
• W1979467260 sameAs 1979467260 @default.
• W1979467260 citedByCount "64" @default.
• W1979467260 countsByYear W19794672602013 @default.
• W1979467260 countsByYear W19794672602014 @default.
• W1979467260 countsByYear W19794672602015 @default.
• W1979467260 countsByYear W19794672602016 @default.
• W1979467260 countsByYear W19794672602017 @default.
• W1979467260 countsByYear W19794672602018 @default.
• W1979467260 countsByYear W19794672602019 @default.
• W1979467260 countsByYear W19794672602020 @default.
• W1979467260 countsByYear W19794672602021 @default.
• W1979467260 countsByYear W19794672602022 @default.
• W1979467260 countsByYear W19794672602023 @default.
• W1979467260 crossrefType "journal-article" @default.
• W1979467260 hasAuthorship W1979467260A5003847961 @default.
• W1979467260 hasAuthorship W1979467260A5057356551 @default.
• W1979467260 hasBestOaLocation W19794672602 @default.
• W1979467260 hasConcept C131779359 @default.
• W1979467260 hasConcept C178790620 @default.
• W1979467260 hasConcept C181199279 @default.
• W1979467260 hasConcept C185592680 @default.
• W1979467260 hasConcept C204921945 @default.
• W1979467260 hasConcept C21951064 @default.
• W1979467260 hasConcept C2775997276 @default.
• W1979467260 hasConcept C2775998212 @default.
• W1979467260 hasConcept C2776016237 @default.
• W1979467260 hasConcept C2778439535 @default.
• W1979467260 hasConcept C2779201268 @default.
• W1979467260 hasConcept C2781208047 @default.
• W1979467260 hasConcept C2781338088 @default.
• W1979467260 hasConcept C40875361 @default.
• W1979467260 hasConcept C47701112 @default.
• W1979467260 hasConcept C515207424 @default.
• W1979467260 hasConcept C521977710 @default.
• W1979467260 hasConcept C55493867 @default.
• W1979467260 hasConcept C71240020 @default.
• W1979467260 hasConceptScore W1979467260C131779359 @default.
• W1979467260 hasConceptScore W1979467260C178790620 @default.
• W1979467260 hasConceptScore W1979467260C181199279 @default.
• W1979467260 hasConceptScore W1979467260C185592680 @default.
• W1979467260 hasConceptScore W1979467260C204921945 @default.
• W1979467260 hasConceptScore W1979467260C21951064 @default.
• W1979467260 hasConceptScore W1979467260C2775997276 @default.
• W1979467260 hasConceptScore W1979467260C2775998212 @default.
• W1979467260 hasConceptScore W1979467260C2776016237 @default.
• W1979467260 hasConceptScore W1979467260C2778439535 @default.
• W1979467260 hasConceptScore W1979467260C2779201268 @default.
• W1979467260 hasConceptScore W1979467260C2781208047 @default.
• W1979467260 hasConceptScore W1979467260C2781338088 @default.
• W1979467260 hasConceptScore W1979467260C40875361 @default.
• W1979467260 hasConceptScore W1979467260C47701112 @default.
• W1979467260 hasConceptScore W1979467260C515207424 @default.
• W1979467260 hasConceptScore W1979467260C521977710 @default.
• W1979467260 hasConceptScore W1979467260C55493867 @default.
• W1979467260 hasConceptScore W1979467260C71240020 @default.
• W1979467260 hasIssue "6" @default.
• W1979467260 hasLocation W19794672601 @default.
• W1979467260 hasLocation W19794672602 @default.
• W1979467260 hasLocation W19794672603 @default.
• W1979467260 hasLocation W19794672604 @default.
• W1979467260 hasOpenAccess W1979467260 @default.
• W1979467260 hasPrimaryLocation W19794672601 @default.
• W1979467260 hasRelatedWork W1979467260 @default.
• W1979467260 hasRelatedWork W1981078824 @default.
• W1979467260 hasRelatedWork W2017159870 @default.
• W1979467260 hasRelatedWork W2728568651 @default.
• W1979467260 hasRelatedWork W2885831566 @default.
• W1979467260 hasRelatedWork W2898101991 @default.
• W1979467260 hasRelatedWork W2949802403 @default.
• W1979467260 hasRelatedWork W4244071240 @default.
• W1979467260 hasRelatedWork W4312192632 @default.

• next