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• W1979536696 endingPage "2034" @default.
• W1979536696 startingPage "2025" @default.
• W1979536696 abstract "Fluorescence detection and imaging are vital technologies in the life sciences and clinical diagnostics. The key to obtaining high-resolution images and sensitive detection is to use fluorescent molecules or particles that absorb and emit visible light with high efficiency. We have synthesized supramolecular complexes consisting of a branched DNA template and fluorogenic intercalating dyes. Because dyes can intercalate up to every other base pair, high densities of fluorophores are assembled yet the DNA template keeps them far enough away from each other to prevent self-quenching. The efficiency with which these noncovalent assemblies absorb light is more than 10-fold greater than that of the individual dye molecules. Förster resonance energy transfer from the intercalated dyes to covalently attached acceptor dyes is very efficient, allowing for wavelength shifting of the emission spectrum. Simple biotinylation of the DNA template allows for labeling of streptavidin-coated synthetic microspheres and mouse T-cells." @default.
• W1979536696 created "2016-06-24" @default.
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• W1979536696 date "2007-01-26" @default.
• W1979536696 modified "2023-09-23" @default.
• W1979536696 title "Fluorescent DNA Nanotags: Supramolecular Fluorescent Labels Based on Intercalating Dye Arrays Assembled on Nanostructured DNA Templates" @default.
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• W1979536696 doi "https://doi.org/10.1021/ja066354t" @default.
• W1979536696 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2532517" @default.
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• W1979536696 hasPublicationYear "2007" @default.
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