Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979621973> ?p ?o ?g. }
- W1979621973 endingPage "872" @default.
- W1979621973 startingPage "868" @default.
- W1979621973 abstract "Objectives To demonstrate that human smooth muscle cells derived from neurogenic bladders produce more collagen in vitro than smooth muscle cells derived from normal bladders, and that epigenetic therapy may normalize this increased collagen production. Methods Human smooth muscle cells from normal (n = 3) and neurogenic bladders (n = 3) were cultured in normal culture media and at different concentrations of the histone deacetylase inhibitors trichostatin A, valproic acid, and the DNA methylation inhibitor 5-azacytidine (5-aza). Collagen type I and III gene expression was measured using real-time quantitative reverse transcription-polymerase chain reaction after varying doses of drug exposure. Cell viability was measured using trypan blue. Results The smooth muscle cells from neurogenic bladders released significantly more collagen than the normal bladder cells (mean 4.1 vs 1.8 μg/mL in control media) when grown in normal conditions. Treatment with trichostatin A at 50 ng/mL decreased the collagen level in cells from neurogenic bladders to almost normal levels (2.1 μg/mL). In addition, valproic acid treatment decreased collagen types I and III gene expression relative to controls, with maximal effect at 300 mg/mL. These treatments had little effect on cell viability. Conclusions Histone deacetylase inhibitors decreased collagen production of smooth muscle cells from neurogenic bladders in vitro. These agents may be a means of effectively preventing bladder fibrosis in patients with this condition. To demonstrate that human smooth muscle cells derived from neurogenic bladders produce more collagen in vitro than smooth muscle cells derived from normal bladders, and that epigenetic therapy may normalize this increased collagen production. Human smooth muscle cells from normal (n = 3) and neurogenic bladders (n = 3) were cultured in normal culture media and at different concentrations of the histone deacetylase inhibitors trichostatin A, valproic acid, and the DNA methylation inhibitor 5-azacytidine (5-aza). Collagen type I and III gene expression was measured using real-time quantitative reverse transcription-polymerase chain reaction after varying doses of drug exposure. Cell viability was measured using trypan blue. The smooth muscle cells from neurogenic bladders released significantly more collagen than the normal bladder cells (mean 4.1 vs 1.8 μg/mL in control media) when grown in normal conditions. Treatment with trichostatin A at 50 ng/mL decreased the collagen level in cells from neurogenic bladders to almost normal levels (2.1 μg/mL). In addition, valproic acid treatment decreased collagen types I and III gene expression relative to controls, with maximal effect at 300 mg/mL. These treatments had little effect on cell viability. Histone deacetylase inhibitors decreased collagen production of smooth muscle cells from neurogenic bladders in vitro. These agents may be a means of effectively preventing bladder fibrosis in patients with this condition." @default.
- W1979621973 created "2016-06-24" @default.
- W1979621973 creator A5006448509 @default.
- W1979621973 creator A5007726050 @default.
- W1979621973 creator A5016629990 @default.
- W1979621973 creator A5082979004 @default.
- W1979621973 date "2010-04-01" @default.
- W1979621973 modified "2023-09-27" @default.
- W1979621973 title "The Effect of Epigenetic Therapy on Congenital Neurogenic Bladders—A Pilot Study" @default.
- W1979621973 cites W103743773 @default.
- W1979621973 cites W1977922402 @default.
- W1979621973 cites W1986159441 @default.
- W1979621973 cites W1992830759 @default.
- W1979621973 cites W2002959213 @default.
- W1979621973 cites W2002974168 @default.
- W1979621973 cites W2005500807 @default.
- W1979621973 cites W2022504623 @default.
- W1979621973 cites W2056047130 @default.
- W1979621973 cites W2060023322 @default.
- W1979621973 cites W2064451566 @default.
- W1979621973 cites W2068500158 @default.
- W1979621973 cites W2081195534 @default.
- W1979621973 cites W2107277218 @default.
- W1979621973 cites W2151705326 @default.
- W1979621973 cites W2155381616 @default.
- W1979621973 cites W2472184467 @default.
- W1979621973 cites W3191441081 @default.
- W1979621973 cites W4318751520 @default.
- W1979621973 doi "https://doi.org/10.1016/j.urology.2009.11.022" @default.
- W1979621973 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2860424" @default.
- W1979621973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20138341" @default.
- W1979621973 hasPublicationYear "2010" @default.
- W1979621973 type Work @default.
- W1979621973 sameAs 1979621973 @default.
- W1979621973 citedByCount "19" @default.
- W1979621973 countsByYear W19796219732012 @default.
- W1979621973 countsByYear W19796219732013 @default.
- W1979621973 countsByYear W19796219732014 @default.
- W1979621973 countsByYear W19796219732015 @default.
- W1979621973 countsByYear W19796219732016 @default.
- W1979621973 countsByYear W19796219732017 @default.
- W1979621973 countsByYear W19796219732019 @default.
- W1979621973 countsByYear W19796219732021 @default.
- W1979621973 countsByYear W19796219732022 @default.
- W1979621973 countsByYear W19796219732023 @default.
- W1979621973 crossrefType "journal-article" @default.
- W1979621973 hasAuthorship W1979621973A5006448509 @default.
- W1979621973 hasAuthorship W1979621973A5007726050 @default.
- W1979621973 hasAuthorship W1979621973A5016629990 @default.
- W1979621973 hasAuthorship W1979621973A5082979004 @default.
- W1979621973 hasBestOaLocation W19796219732 @default.
- W1979621973 hasConcept C104317684 @default.
- W1979621973 hasConcept C142724271 @default.
- W1979621973 hasConcept C153911025 @default.
- W1979621973 hasConcept C202751555 @default.
- W1979621973 hasConcept C2776202225 @default.
- W1979621973 hasConcept C2777865847 @default.
- W1979621973 hasConcept C2778305200 @default.
- W1979621973 hasConcept C2780644872 @default.
- W1979621973 hasConcept C41091548 @default.
- W1979621973 hasConcept C53227056 @default.
- W1979621973 hasConcept C552990157 @default.
- W1979621973 hasConcept C55493867 @default.
- W1979621973 hasConcept C64927066 @default.
- W1979621973 hasConcept C71924100 @default.
- W1979621973 hasConcept C86803240 @default.
- W1979621973 hasConceptScore W1979621973C104317684 @default.
- W1979621973 hasConceptScore W1979621973C142724271 @default.
- W1979621973 hasConceptScore W1979621973C153911025 @default.
- W1979621973 hasConceptScore W1979621973C202751555 @default.
- W1979621973 hasConceptScore W1979621973C2776202225 @default.
- W1979621973 hasConceptScore W1979621973C2777865847 @default.
- W1979621973 hasConceptScore W1979621973C2778305200 @default.
- W1979621973 hasConceptScore W1979621973C2780644872 @default.
- W1979621973 hasConceptScore W1979621973C41091548 @default.
- W1979621973 hasConceptScore W1979621973C53227056 @default.
- W1979621973 hasConceptScore W1979621973C552990157 @default.
- W1979621973 hasConceptScore W1979621973C55493867 @default.
- W1979621973 hasConceptScore W1979621973C64927066 @default.
- W1979621973 hasConceptScore W1979621973C71924100 @default.
- W1979621973 hasConceptScore W1979621973C86803240 @default.
- W1979621973 hasIssue "4" @default.
- W1979621973 hasLocation W19796219731 @default.
- W1979621973 hasLocation W19796219732 @default.
- W1979621973 hasLocation W19796219733 @default.
- W1979621973 hasLocation W19796219734 @default.
- W1979621973 hasOpenAccess W1979621973 @default.
- W1979621973 hasPrimaryLocation W19796219731 @default.
- W1979621973 hasRelatedWork W2012619750 @default.
- W1979621973 hasRelatedWork W2029788469 @default.
- W1979621973 hasRelatedWork W2042377847 @default.
- W1979621973 hasRelatedWork W2053139375 @default.
- W1979621973 hasRelatedWork W2085913918 @default.
- W1979621973 hasRelatedWork W2154639279 @default.
- W1979621973 hasRelatedWork W2511725000 @default.
- W1979621973 hasRelatedWork W2552712024 @default.
- W1979621973 hasRelatedWork W2563611942 @default.
- W1979621973 hasRelatedWork W2791494815 @default.