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- W1979642806 abstract "Estrone and estradiol-17β have been measured in non-pregnancy plasma, by radioimmunoassay, after Chromatographie separation on short Sephadex LH20 columns. Dextran coated charcoal has been used for adsorbing unbound estrogen in the incubation mixture. No meaningful results could be obtained by measurement of total free estrogens in crude extracts of non-pregnancy plasma. In non-pregnancy plasma, the limited specificity of the antibodies imposed the chromatography step. The precision of the measurements during the menstrual cycle was of 9–15% (coefficient of variation). In pregnancy plasma the direct measurement of free estrogens in crude extracts, without chromatography, gave results that were proportional to the volume of plasma extracted. The precision of the measurements varied between 7–15% (coefficient of variation), for both low and high plasma concentration. In pregnant subjects a good correlation was found between the level of unconjugated estrogens in the plasma and the level of urinary estriol." @default.
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- W1979642806 date "1973-01-01" @default.
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- W1979642806 title "Radioimmunoassay of estrone and estradiol-17β in peripheral plasma of pregnant and non-pregnant women" @default.
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- W1979642806 doi "https://doi.org/10.1016/0022-4731(73)90082-4" @default.
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