FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979653803> ?p ?o ?g. }

• W1979653803 endingPage "13059" @default.
• W1979653803 startingPage "13051" @default.
• W1979653803 abstract "Bacterial flagella are turned by rotary motors that obtain energy from the membrane gradient of protons or sodium ions. The stator of the flagellar motor is formed from the membrane proteins MotA and MotB, which associate in complexes that contain multiple copies of each protein. The complexes conduct ions across the membrane, and couple ion flow to motor rotation by a mechanism that appears to involve conformational changes [Kojima, S., and Blair, D. F. (2001) Biochemistry 40, 13041-13050]. Structural information on the MotA/MotB complex is very limited. MotA has four membrane-spanning segments, and MotB has one. We have begun a targeted disulfide-cross-linking study to probe the arrangement of membrane segments in the MotA/MotB complex, beginning with the single membrane segment of MotB. Cys residues were introduced in 21 consecutive positions in the segment, and disulfide cross-linking was studied in MotA/MotB complexes either in membranes or detergent solution. Most of the Cys-substituted MotB proteins formed disulfide-linked dimers in significant yield upon oxidation. The yield of dimer varied regularly with the position of the Cys substitution, following the pattern expected for a parallel, symmetric dimer of alpha-helices. In a structural model based on the cross-linking experiments, critical Asp32 residues that are believed to facilitate proton movement are positioned on separate surfaces of the MotB dimer and so probably function within two distinct proton channels. Regions accessible to solvent were mapped by measuring the reactivity of introduced Cys residues toward N-ethyl maleimide and a charged methanethiosulfonate reagent. Positions near the middle of the segment were inaccessible to sulhydryl reagents. Positions within 6-8 residues of either end, which includes residues around Asp32, were accessible." @default.
• W1979653803 created "2016-06-24" @default.
• W1979653803 creator A5011059944 @default.
• W1979653803 creator A5079201187 @default.
• W1979653803 date "2001-10-01" @default.
• W1979653803 modified "2023-09-25" @default.
• W1979653803 title "Targeted Disulfide Cross-Linking of the MotB Protein of <i>Escherichia</i> <i>coli</i>: Evidence for Two H⁺ Channels in the Stator Complex" @default.
• W1979653803 cites W1536544212 @default.
• W1979653803 cites W1595581340 @default.
• W1979653803 cites W1920217070 @default.
• W1979653803 cites W1975993749 @default.
• W1979653803 cites W1978807168 @default.
• W1979653803 cites W1994390600 @default.
• W1979653803 cites W2005388968 @default.
• W1979653803 cites W2084205512 @default.
• W1979653803 cites W2147233352 @default.
• W1979653803 cites W2158585966 @default.
• W1979653803 cites W2166841699 @default.
• W1979653803 cites W2172608051 @default.
• W1979653803 doi "https://doi.org/10.1021/bi011264g" @default.
• W1979653803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11669643" @default.
• W1979653803 hasPublicationYear "2001" @default.
• W1979653803 type Work @default.
• W1979653803 sameAs 1979653803 @default.
• W1979653803 citedByCount "117" @default.
• W1979653803 countsByYear W19796538032012 @default.
• W1979653803 countsByYear W19796538032013 @default.
• W1979653803 countsByYear W19796538032014 @default.
• W1979653803 countsByYear W19796538032015 @default.
• W1979653803 countsByYear W19796538032016 @default.
• W1979653803 countsByYear W19796538032017 @default.
• W1979653803 countsByYear W19796538032018 @default.
• W1979653803 countsByYear W19796538032019 @default.
• W1979653803 countsByYear W19796538032020 @default.
• W1979653803 countsByYear W19796538032021 @default.
• W1979653803 countsByYear W19796538032022 @default.
• W1979653803 countsByYear W19796538032023 @default.
• W1979653803 crossrefType "journal-article" @default.
• W1979653803 hasAuthorship W1979653803A5011059944 @default.
• W1979653803 hasAuthorship W1979653803A5079201187 @default.
• W1979653803 hasConcept C104317684 @default.
• W1979653803 hasConcept C115811362 @default.
• W1979653803 hasConcept C12554922 @default.
• W1979653803 hasConcept C144647389 @default.
• W1979653803 hasConcept C145148216 @default.
• W1979653803 hasConcept C178790620 @default.
• W1979653803 hasConcept C185592680 @default.
• W1979653803 hasConcept C2779546866 @default.
• W1979653803 hasConcept C41625074 @default.
• W1979653803 hasConcept C547475151 @default.
• W1979653803 hasConcept C55493867 @default.
• W1979653803 hasConcept C8010536 @default.
• W1979653803 hasConcept C86803240 @default.
• W1979653803 hasConcept C90424259 @default.
• W1979653803 hasConceptScore W1979653803C104317684 @default.
• W1979653803 hasConceptScore W1979653803C115811362 @default.
• W1979653803 hasConceptScore W1979653803C12554922 @default.
• W1979653803 hasConceptScore W1979653803C144647389 @default.
• W1979653803 hasConceptScore W1979653803C145148216 @default.
• W1979653803 hasConceptScore W1979653803C178790620 @default.
• W1979653803 hasConceptScore W1979653803C185592680 @default.
• W1979653803 hasConceptScore W1979653803C2779546866 @default.
• W1979653803 hasConceptScore W1979653803C41625074 @default.
• W1979653803 hasConceptScore W1979653803C547475151 @default.
• W1979653803 hasConceptScore W1979653803C55493867 @default.
• W1979653803 hasConceptScore W1979653803C8010536 @default.
• W1979653803 hasConceptScore W1979653803C86803240 @default.
• W1979653803 hasConceptScore W1979653803C90424259 @default.
• W1979653803 hasIssue "43" @default.
• W1979653803 hasLocation W19796538031 @default.
• W1979653803 hasLocation W19796538032 @default.
• W1979653803 hasOpenAccess W1979653803 @default.
• W1979653803 hasPrimaryLocation W19796538031 @default.
• W1979653803 hasRelatedWork W1708232870 @default.
• W1979653803 hasRelatedWork W1824016493 @default.
• W1979653803 hasRelatedWork W1916445119 @default.
• W1979653803 hasRelatedWork W1971558825 @default.
• W1979653803 hasRelatedWork W1973366735 @default.
• W1979653803 hasRelatedWork W1988737860 @default.
• W1979653803 hasRelatedWork W2044402419 @default.
• W1979653803 hasRelatedWork W2067006121 @default.
• W1979653803 hasRelatedWork W2950036561 @default.
• W1979653803 hasRelatedWork W2950776771 @default.
• W1979653803 hasVolume "40" @default.
• W1979653803 isParatext "false" @default.
• W1979653803 isRetracted "false" @default.
• W1979653803 magId "1979653803" @default.
• W1979653803 workType "article" @default.