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• W1979687905 abstract "The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isothiocyanate, E.Z.N.A. Mollusc Kit, Chelex-100, and lysis with detergents) were compared. A nested PCR method combined with 3.5 h pre-enrichment in buffered peptone water (BPW) and DNA extraction by the resin Chelex-100 is proposed for the detection of S. senftenberg in oyster samples. The detection limit of the method is less than 0.1 CFU/ml (<1 CFU/g of oyster). This procedure is shown to be an excellent tool for the sensitive detection of S. senftenberg from naturally contaminated oysters, with results being obtained within 8 h." @default.
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• W1979687905 date "2005-02-01" @default.
• W1979687905 modified "2023-10-12" @default.
• W1979687905 title "Eight-hour PCR-based procedure for the detection of<i>Salmonella</i>in raw oysters" @default.
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• W1979687905 doi "https://doi.org/10.1016/j.femsle.2004.12.016" @default.
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