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• W1979690999 abstract "Ziehl-Neelsen (ZN) staining is the key technique for diagnosis of mycobacterial infections; however, a high percentage of patients exhibit positive signs of tuberculosis, as indicated by pathology, culture of mycobacteria, and polymerase chain-reaction analysis, and yet show negative results on ZN staining. In this report we present evidence that such ZN-negative specimens represent Mycobacterium tuberculosis bacilli in a dormant state with distinct cell-wall alterations: the classical cell-wall composition-dependent ZN staining of M. tuberculosis in lung sections gradually discontinued with persistence of infection, both in mice and in human patients; in contrast, detection of mycobacteria by cell-wall composition-independent staining using a polyclonal anti-M. bovis Bacille-Calmette-Guérin serum continued with persistence of infection. These findings have important implications for diagnosis, as well as for both chemotherapy and development of vaccine strategies.'" @default.
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• W1979690999 date "2003-11-01" @default.
• W1979690999 modified "2023-10-17" @default.
• W1979690999 title "Cell‐Wall Alterations as an Attribute of<i>Mycobacterium tuberculosis</i>in Latent Infection" @default.
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• W1979690999 doi "https://doi.org/10.1086/378563" @default.
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