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- W1979813992 abstract "HYDROPEROXIDE LYASE (HPL) genes encode enzymes that catalyze the cleavage of fatty acid hydroperoxides into aldehydesand oxoacids. There are three HPLs in rice (Oryza sativa), designated OsHPL1 through OsHPL3. To explore the possibility ofdifferential functional activities among these genes, we have examined their expression patterns and biochemical properties oftheir encoded products. Transcript analysis indicates that these genes have distinct patterns and levels of expression. OsHPL1is ubiquitously expressed, OsHPL2 is expressed in the leaves and leaf sheaths, whereas OsHPL3 is wound inducible andexpressed exclusively in leaves. OsHPLs also differ in their substrate preference as determined by in vitro enzyme assays using9-/13-hydroperoxy linolenic and 9-/13-hydroperoxy linoleic acids as substrates. OsHPL1 and OsHPL2 metabolize 9-/13-hydroperoxides, whereas OsHPL3 metabolizes 13-hydroperoxy linolenic acid exclusively. Sequence alignments of the HPLenzymes have identified signature residues potentially responsible for the substrate specificity/preference of these enzymes.All three OsHPLs are chloroplast localized as determined by chloroplast import assays and green fluorescent protein (GFP)fusion studies. Aldehyde measurements in transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing individualOsHPL-GFP fusions indicate that all rice HPLs are functional in a heterologous system, and each of them generates a distinctsignature of the metabolites. Interestingly, these aldehydes were only detectable in leaves, but not in roots, despite similarlevels of OsHPL-GFP proteins in both tissues. Similarly, there were undetectable levels of aldehydes in rice roots, in spite of thepresence of OsHPL1 transcripts. Together, these data suggest that additional tissue-specific mechanism(s) beyond transcriptand HPL enzyme abundance, regulate the levels of HPL-derived metabolites." @default.
- W1979813992 created "2016-06-24" @default.
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- W1979813992 date "2006-01-01" @default.
- W1979813992 modified "2023-09-24" @default.
- W1979813992 title "Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1" @default.
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