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- W1979822078 abstract "A DNA probe obtained from plasmid pBR 322 was developed for use in a ‘colony hybridization’ method with crude bacterial lysates to detect TEM-1 and TEM-2 β-lactamases. Biotin-labeled and digoxigenin-labeled probes were compared with isoelectric focusing (IEF) for the detection of TEM-1 and TEM-2 β-lactamases in 180 clinical isolates of various Gram-negative bacterial species. 89 (49.4%) isolates contained TEM-type enzymes, as determined by IEF, all reacted with the biotin-labeled probe. However, the remaining 91 isolates also hybridized with this probe, despite the fact that 62 isolates had no detectable β-lactamase activity in a hydrolytic assay. In contrast, the digoxigenin-labeled probe showed hybridization with 75/89 (84%) of the TEM-containing isolates and this probe reacted only with 2 of 29 (7%) isolates positiv for other β-lactamases and 3/62 (4.8%) isolates with no hydrolytic activity. We conclude that the digoxinenin-labeled probe was more specific than its biotin-labeled counterpart and its sensitivity was only marginally less than IEF for the detection of TEM-type-β-lactamases." @default.
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- W1979822078 date "1990-07-01" @default.
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- W1979822078 title "Digoxigenin-labeled DNA probe to detect TEM type β-lactamases" @default.
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- W1979822078 doi "https://doi.org/10.1016/0167-7012(90)90063-c" @default.
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