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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979842004> ?p ?o ?g. }

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• W1979842004 abstract "Melanin-concentrating hormone (MCH), which is an orexigenic peptide, was isolated and identified as the endogenous ligand of the SLC-1 receptor. We established a CHO cell line expressing the rat SLC-1 receptor to search for its endogenous ligand. The extract of rat whole brain showed inhibition of intracellular forskolin-induced cAMP accumulation in rat SLC-1-expressing CHO cells and was purified. Using HPLC purification, we isolated and identified MCH as the endogenous ligand of the SLC-1 receptor. The authentic MCH demonstrated a dose-dependent inhibitory effect on cAMP accumulation in forskolin-stimulated rat and human SLC-1-expressing CHO cells with an EC50 value of 0.2 nM for both the rat and human SLC-1 receptors. This is the first description of the functional receptor for MCH." @default.
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• W1979842004 date "1999-08-01" @default.
• W1979842004 modified "2023-09-23" @default.
• W1979842004 title "Isolation and Identification of Melanin-Concentrating Hormone as the Endogenous Ligand of the SLC-1 Receptor" @default.
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• W1979842004 doi "https://doi.org/10.1006/bbrc.1999.1104" @default.
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