Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979851859> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W1979851859 endingPage "467" @default.
- W1979851859 startingPage "451" @default.
- W1979851859 abstract "Orotic acid phosphoribosyltransferase (EC 2.4.2.10) and orotidylate decarboxylase (EC 4.1.1.23) can be assayed independently of one another by the high voltage paper electrophoresis method described here, which separates orotic acid, OMP, and UMP, the substrates and/or products of these enzymes, from each other. The relative migration of other compounds, mainly other nucleotides, their bases, or other intermediates of the UMP biosynthetic pathway, has also been recorded. The method has allowed us to observe that OMP is not released to any significant degree from the enzyme complex of these two enzymes that occurs in Ehrlich ascites cells; rather orotic acid is converted stoichiometrically by the enzyme complex to UMP. For purification of the enzyme complex, we have found the release of 14CO2 from [14C]carboxyl-labeled orotic acid (when phosphoribosyl pyrophosphate and Mg2+ are present) preferable to the HVPE method as a routine assay procedure. The most economical CO2-absorbant for the assay of the enzyme complex or for orotidylate decarboxylase (and possibly for other enzymes which release CO2) is an NaOH-soaked paper strip. As detailed here, its use allows one to repeatedly reuse the scintillation vials and fluid." @default.
- W1979851859 created "2016-06-24" @default.
- W1979851859 creator A5029417472 @default.
- W1979851859 creator A5089034302 @default.
- W1979851859 date "1975-12-01" @default.
- W1979851859 modified "2023-10-18" @default.
- W1979851859 title "Radioassay of orotic acid phosphoribosyltransferase and orotidylate decarboxylase utilizing a high-voltage paper electrophoresis technique or an improved 14CO2-release method" @default.
- W1979851859 cites W105378468 @default.
- W1979851859 cites W137087687 @default.
- W1979851859 cites W1515500403 @default.
- W1979851859 cites W1546005878 @default.
- W1979851859 cites W1593424026 @default.
- W1979851859 cites W180758481 @default.
- W1979851859 cites W185758696 @default.
- W1979851859 cites W1977066380 @default.
- W1979851859 cites W2024402313 @default.
- W1979851859 cites W2034627702 @default.
- W1979851859 cites W2036778327 @default.
- W1979851859 cites W204227082 @default.
- W1979851859 cites W2047171091 @default.
- W1979851859 cites W2086605057 @default.
- W1979851859 cites W2094733018 @default.
- W1979851859 cites W2144043129 @default.
- W1979851859 cites W4233174300 @default.
- W1979851859 doi "https://doi.org/10.1016/0003-2697(75)90147-5" @default.
- W1979851859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1217711" @default.
- W1979851859 hasPublicationYear "1975" @default.
- W1979851859 type Work @default.
- W1979851859 sameAs 1979851859 @default.
- W1979851859 citedByCount "41" @default.
- W1979851859 crossrefType "journal-article" @default.
- W1979851859 hasAuthorship W1979851859A5029417472 @default.
- W1979851859 hasAuthorship W1979851859A5089034302 @default.
- W1979851859 hasConcept C104317684 @default.
- W1979851859 hasConcept C181199279 @default.
- W1979851859 hasConcept C185592680 @default.
- W1979851859 hasConcept C2775851010 @default.
- W1979851859 hasConcept C2776476023 @default.
- W1979851859 hasConcept C40250595 @default.
- W1979851859 hasConcept C40507961 @default.
- W1979851859 hasConcept C43617362 @default.
- W1979851859 hasConcept C512185932 @default.
- W1979851859 hasConcept C55493867 @default.
- W1979851859 hasConceptScore W1979851859C104317684 @default.
- W1979851859 hasConceptScore W1979851859C181199279 @default.
- W1979851859 hasConceptScore W1979851859C185592680 @default.
- W1979851859 hasConceptScore W1979851859C2775851010 @default.
- W1979851859 hasConceptScore W1979851859C2776476023 @default.
- W1979851859 hasConceptScore W1979851859C40250595 @default.
- W1979851859 hasConceptScore W1979851859C40507961 @default.
- W1979851859 hasConceptScore W1979851859C43617362 @default.
- W1979851859 hasConceptScore W1979851859C512185932 @default.
- W1979851859 hasConceptScore W1979851859C55493867 @default.
- W1979851859 hasIssue "2" @default.
- W1979851859 hasLocation W19798518591 @default.
- W1979851859 hasLocation W19798518592 @default.
- W1979851859 hasOpenAccess W1979851859 @default.
- W1979851859 hasPrimaryLocation W19798518591 @default.
- W1979851859 hasRelatedWork W1963809934 @default.
- W1979851859 hasRelatedWork W1997683263 @default.
- W1979851859 hasRelatedWork W1997741528 @default.
- W1979851859 hasRelatedWork W2013157260 @default.
- W1979851859 hasRelatedWork W2016209517 @default.
- W1979851859 hasRelatedWork W2054430652 @default.
- W1979851859 hasRelatedWork W2066243052 @default.
- W1979851859 hasRelatedWork W2094074402 @default.
- W1979851859 hasRelatedWork W2110152851 @default.
- W1979851859 hasRelatedWork W2080271760 @default.
- W1979851859 hasVolume "69" @default.
- W1979851859 isParatext "false" @default.
- W1979851859 isRetracted "false" @default.
- W1979851859 magId "1979851859" @default.
- W1979851859 workType "article" @default.