FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979968256> ?p ?o ?g. }

• W1979968256 endingPage "281" @default.
• W1979968256 startingPage "276" @default.
• W1979968256 abstract "Tubulin-tyrosine ligase (TTL), the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin, has been purified from porcine brain using a purification scheme based on standard biochemical procedures. The enzyme preparation was nearly homogeneous (purity greater than 95%), was free of tubulin, and could be stored in the presence of glycerol for several months without loss in activity. To develop a more convenient purification of TTL, we have isolated mouse hybridoma cells secreting antibodies to TTL. These monoclonal antibodies recognize TTL not only in brain tissue but also in the liver of various mammals. Monoclonal antibodies isolated from ascites fluid allowed a rapid purification of TTL from a crude brain extract. TTL stayed bound to the immunoaffinity column in 1.5 M NaCl and was eluted with 3 M MgCl2. Highly active TTL was recovered nearly quantitatively at greater than 95% purity and could be stabilized in the presence of glycerol. Glycerol gradient centrifugation, SDS gel electrophoresis and immunoblots identified TTL as a monomeric protein with an apparent polypeptide molecular weight of about 40,000. A one to one complex of TTL with alpha beta-tubulin was observed by gradient centrifugation." @default.
• W1979968256 created "2016-06-24" @default.
• W1979968256 creator A5052210547 @default.
• W1979968256 creator A5054146037 @default.
• W1979968256 creator A5086108824 @default.
• W1979968256 date "1985-01-01" @default.
• W1979968256 modified "2023-09-23" @default.
• W1979968256 title "Purification of brain tubulin-tyrosine ligase by biochemical and immunological methods." @default.
• W1979968256 cites W116661026 @default.
• W1979968256 cites W1419804308 @default.
• W1979968256 cites W1443907896 @default.
• W1979968256 cites W1469270584 @default.
• W1979968256 cites W1611482599 @default.
• W1979968256 cites W1772857359 @default.
• W1979968256 cites W1775749144 @default.
• W1979968256 cites W1933510681 @default.
• W1979968256 cites W1967804636 @default.
• W1979968256 cites W1978945144 @default.
• W1979968256 cites W2008636495 @default.
• W1979968256 cites W2013757942 @default.
• W1979968256 cites W2014636427 @default.
• W1979968256 cites W2018289835 @default.
• W1979968256 cites W2035422766 @default.
• W1979968256 cites W2051805967 @default.
• W1979968256 cites W2053069944 @default.
• W1979968256 cites W2056218052 @default.
• W1979968256 cites W2059465841 @default.
• W1979968256 cites W2059581106 @default.
• W1979968256 cites W2087062084 @default.
• W1979968256 cites W2100837269 @default.
• W1979968256 cites W2101108802 @default.
• W1979968256 cites W2133128874 @default.
• W1979968256 cites W2163699431 @default.
• W1979968256 cites W255194224 @default.
• W1979968256 cites W32245353 @default.
• W1979968256 cites W48964459 @default.
• W1979968256 cites W983448995 @default.
• W1979968256 doi "https://doi.org/10.1083/jcb.100.1.276" @default.
• W1979968256 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2113468" @default.
• W1979968256 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3965474" @default.
• W1979968256 hasPublicationYear "1985" @default.
• W1979968256 type Work @default.
• W1979968256 sameAs 1979968256 @default.
• W1979968256 citedByCount "70" @default.
• W1979968256 countsByYear W19799682562012 @default.
• W1979968256 countsByYear W19799682562014 @default.
• W1979968256 countsByYear W19799682562015 @default.
• W1979968256 countsByYear W19799682562016 @default.
• W1979968256 countsByYear W19799682562017 @default.
• W1979968256 countsByYear W19799682562018 @default.
• W1979968256 countsByYear W19799682562019 @default.
• W1979968256 countsByYear W19799682562020 @default.
• W1979968256 countsByYear W19799682562021 @default.
• W1979968256 countsByYear W19799682562022 @default.
• W1979968256 countsByYear W19799682562023 @default.
• W1979968256 crossrefType "journal-article" @default.
• W1979968256 hasAuthorship W1979968256A5052210547 @default.
• W1979968256 hasAuthorship W1979968256A5054146037 @default.
• W1979968256 hasAuthorship W1979968256A5086108824 @default.
• W1979968256 hasBestOaLocation W19799682561 @default.
• W1979968256 hasConcept C153911025 @default.
• W1979968256 hasConcept C159654299 @default.
• W1979968256 hasConcept C175114707 @default.
• W1979968256 hasConcept C181199279 @default.
• W1979968256 hasConcept C185592680 @default.
• W1979968256 hasConcept C203014093 @default.
• W1979968256 hasConcept C20418707 @default.
• W1979968256 hasConcept C2776165026 @default.
• W1979968256 hasConcept C2780881558 @default.
• W1979968256 hasConcept C43617362 @default.
• W1979968256 hasConcept C542903549 @default.
• W1979968256 hasConcept C55493867 @default.
• W1979968256 hasConcept C7623868 @default.
• W1979968256 hasConcept C84425145 @default.
• W1979968256 hasConcept C86803240 @default.
• W1979968256 hasConcept C95444343 @default.
• W1979968256 hasConceptScore W1979968256C153911025 @default.
• W1979968256 hasConceptScore W1979968256C159654299 @default.
• W1979968256 hasConceptScore W1979968256C175114707 @default.
• W1979968256 hasConceptScore W1979968256C181199279 @default.
• W1979968256 hasConceptScore W1979968256C185592680 @default.
• W1979968256 hasConceptScore W1979968256C203014093 @default.
• W1979968256 hasConceptScore W1979968256C20418707 @default.
• W1979968256 hasConceptScore W1979968256C2776165026 @default.
• W1979968256 hasConceptScore W1979968256C2780881558 @default.
• W1979968256 hasConceptScore W1979968256C43617362 @default.
• W1979968256 hasConceptScore W1979968256C542903549 @default.
• W1979968256 hasConceptScore W1979968256C55493867 @default.
• W1979968256 hasConceptScore W1979968256C7623868 @default.
• W1979968256 hasConceptScore W1979968256C84425145 @default.
• W1979968256 hasConceptScore W1979968256C86803240 @default.
• W1979968256 hasConceptScore W1979968256C95444343 @default.
• W1979968256 hasIssue "1" @default.
• W1979968256 hasLocation W19799682561 @default.
• W1979968256 hasLocation W19799682562 @default.
• W1979968256 hasLocation W19799682563 @default.
• W1979968256 hasLocation W19799682564 @default.
• W1979968256 hasOpenAccess W1979968256 @default.

• next