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- W1979969439 abstract "Abstract A technic was presented combining direct and indirect immunofluorescent staining (IFS) to identify cells containing anibody to a haptenic group and the immunuoglobulin (Ig) class of that antibody. Fluorescein isothiocyanate or tetramethylrhodamine isothiocyanate were conjugated with IgG prepared from various antisera by precipitation with 1.4 M (NH4)2SO4 and DEAE-cellulose chromatography. The reagent in the first staining step contained rhodamine-conjugated antibodies to a specific Ig class and dinitrophenylated human γ-globulin. After washing, the tissue section was stained with fluorescein-conjugated IgG prepared from an antiserum to dinitrophenylated bovine γ-globulin. Fluorescence microscopy revealed either Ig class or antibody to DNP or both in the same cell. Combined IFS was simpler and required less time to obtain results than other technics previously used to identify antibody and Ig class in a single cell." @default.
- W1979969439 created "2016-06-24" @default.
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- W1979969439 date "1974-10-01" @default.
- W1979969439 modified "2023-09-26" @default.
- W1979969439 title "Combined immunofluorescent staining for the simultaneous detection of anti-DNP antibody and immunoglobulin class in the same cell" @default.
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- W1979969439 doi "https://doi.org/10.1016/0022-1759(74)90016-7" @default.
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