Matches in SemOpenAlex for { <https://semopenalex.org/work/W1980085014> ?p ?o ?g. }
- W1980085014 endingPage "43" @default.
- W1980085014 startingPage "27" @default.
- W1980085014 abstract "Immunogold electron microscopy has identified a variety of blood-brain barrier (BBB) proteins with transporter and regulatory functions. For example, isoforms of the glucose transporter, protein kinase C (PKC), and caveolin-1 are BBB specific. Isoform 1 of the facilitative glucose transporter family (GLUT1) is expressed solely in endothelial (and pericyte) domains, and ∼75% of the protein is membrane-localized in humans. Evidence is presented for a water cotransport function of BBB GLUT1. A shift in transporter polarity characterized by increased luminal membrane GLUT1 is seen when BBB glucose transport is upregulated; but a greater abluminal membrane density is seen in the human BBB when GLUT1 is downregulated. PKC colocalizes with GLUT1 within these endothelial domains during up- and downregulation, suggesting that a PKC-mediated mechanism regulates human BBB glucose transporter expression. Occludin and claudin-5 (like other tight-junctional proteins) exhibit a restricted distribution, and are expressed solely within interendothelial clefts of the BBB. GFAP (glial fibrillary acidic protein) is uniformly expressed throughout the foot-processes and the entire astrocyte. But the microvascular-facing membranes of the glial processes that contact the basal laminae are also polarized, and their transporters may also redistribute within the astrocyte. Monocarboxylic acid transporter and water channel (Aquaporin-4) expression are enriched at the glial foot-process, and both undergo physiological modulation. We suggest that as transcytosis and efflux mechanisms generate interest as potential neurotherapeutic targets, electron microscopic confirmation of their site-specific expression patterns will continue to support the CNS drug discovery process." @default.
- W1980085014 created "2016-06-24" @default.
- W1980085014 creator A5002767613 @default.
- W1980085014 creator A5017625603 @default.
- W1980085014 date "2005-01-01" @default.
- W1980085014 modified "2023-10-13" @default.
- W1980085014 title "Localization of brain endothelial luminal and abluminal transporters with immunogold electron microscopy" @default.
- W1980085014 cites W113678817 @default.
- W1980085014 cites W1484884286 @default.
- W1980085014 cites W1494587882 @default.
- W1980085014 cites W1501069255 @default.
- W1980085014 cites W1532974894 @default.
- W1980085014 cites W1535419310 @default.
- W1980085014 cites W1574043141 @default.
- W1980085014 cites W1575140355 @default.
- W1980085014 cites W1579545702 @default.
- W1980085014 cites W1595319072 @default.
- W1980085014 cites W1607061495 @default.
- W1980085014 cites W1781422850 @default.
- W1980085014 cites W1970392661 @default.
- W1980085014 cites W1971824649 @default.
- W1980085014 cites W1972649549 @default.
- W1980085014 cites W1977371761 @default.
- W1980085014 cites W1977450247 @default.
- W1980085014 cites W1980829307 @default.
- W1980085014 cites W1981956388 @default.
- W1980085014 cites W1985944040 @default.
- W1980085014 cites W1991078537 @default.
- W1980085014 cites W1991091534 @default.
- W1980085014 cites W1993064394 @default.
- W1980085014 cites W1994991276 @default.
- W1980085014 cites W1995793237 @default.
- W1980085014 cites W1996123970 @default.
- W1980085014 cites W1996462589 @default.
- W1980085014 cites W1997382203 @default.
- W1980085014 cites W2001178402 @default.
- W1980085014 cites W2005062241 @default.
- W1980085014 cites W2006070968 @default.
- W1980085014 cites W2006072955 @default.
- W1980085014 cites W2006962652 @default.
- W1980085014 cites W2014009661 @default.
- W1980085014 cites W2016551781 @default.
- W1980085014 cites W2017811372 @default.
- W1980085014 cites W2019569045 @default.
- W1980085014 cites W2020899233 @default.
- W1980085014 cites W2024530909 @default.
- W1980085014 cites W2024796153 @default.
- W1980085014 cites W2025476539 @default.
- W1980085014 cites W2025706831 @default.
- W1980085014 cites W2026834560 @default.
- W1980085014 cites W2027170943 @default.
- W1980085014 cites W2032379027 @default.
- W1980085014 cites W2034763077 @default.
- W1980085014 cites W2035367133 @default.
- W1980085014 cites W2038624271 @default.
- W1980085014 cites W2041705163 @default.
- W1980085014 cites W2042798743 @default.
- W1980085014 cites W2043498565 @default.
- W1980085014 cites W2043632402 @default.
- W1980085014 cites W2043804739 @default.
- W1980085014 cites W2044196139 @default.
- W1980085014 cites W2046653028 @default.
- W1980085014 cites W2046847544 @default.
- W1980085014 cites W2048283588 @default.
- W1980085014 cites W2050285850 @default.
- W1980085014 cites W2050691765 @default.
- W1980085014 cites W2053410982 @default.
- W1980085014 cites W2053525983 @default.
- W1980085014 cites W2055778069 @default.
- W1980085014 cites W2057552727 @default.
- W1980085014 cites W2059368790 @default.
- W1980085014 cites W2063454950 @default.
- W1980085014 cites W2063528544 @default.
- W1980085014 cites W2064631960 @default.
- W1980085014 cites W2072849537 @default.
- W1980085014 cites W2075183125 @default.
- W1980085014 cites W2080139260 @default.
- W1980085014 cites W2081320648 @default.
- W1980085014 cites W2081967898 @default.
- W1980085014 cites W2083083064 @default.
- W1980085014 cites W2083274084 @default.
- W1980085014 cites W2090006669 @default.
- W1980085014 cites W2093313366 @default.
- W1980085014 cites W2101842469 @default.
- W1980085014 cites W2102595439 @default.
- W1980085014 cites W2103467580 @default.
- W1980085014 cites W2104567497 @default.
- W1980085014 cites W2104899166 @default.
- W1980085014 cites W2105112166 @default.
- W1980085014 cites W2106303836 @default.
- W1980085014 cites W2111251868 @default.
- W1980085014 cites W2115515762 @default.
- W1980085014 cites W2120679080 @default.
- W1980085014 cites W2121175414 @default.
- W1980085014 cites W2129323165 @default.
- W1980085014 cites W2130488937 @default.
- W1980085014 cites W2132174291 @default.
- W1980085014 cites W2134666057 @default.