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- W1980096859 abstract "INTRODUCTION This protocol describes a method for in-gel digestion of proteins after fractionation using SDS-PAGE. It is applicable to both one- and two-dimensional polyacrylamide gels of different thicknesses, acrylamide concentrations, and band (spot) sizes. This protocol followed by liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) or matrix-assisted laser desorption/ionization/time of flight/time of flight (MALDI-TOF/TOF) is capable of identifying low femtomole quantities of a protein. High-sensitivity mass spectrometry should be capable of identifying attomole quantities of protein." @default.
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- W1980096859 date "2009-02-01" @default.
- W1980096859 modified "2023-10-02" @default.
- W1980096859 title "In-Gel Trypsin Digest of Gel-Fractionated Proteins" @default.
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- W1980096859 doi "https://doi.org/10.1101/pdb.prot5110" @default.
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