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- W1980102015 abstract "UV-endonuclease from Microcossuc luteus induces single-stranded breaks in UV-irradiated DNA of phage lambda and the average length of the fragments produced (after UV-doses to DNA of 135 and 675 erg/mm2) is equal to the average spacing between pyrimidine dimers. The plaque-forming ability of UV-irradiated lambda DNA used to infect Ca++-treated uvr A6, uvrB5 or uvrC34 recipient Escherichia coli cells (but not uve+ cells) may be significantly enhanced by treatment of lambda DNA with UV-endonuclease. This enzyme strongly decreases the reactivation of UV-irradiated lambda DNA caused by UV-irradiation of uvr+ or uvrA6 Ca++-treated cells and eliminates most clear-mutations especially if mutations are analysed using Ca++-treated uvr A6 recipient cells. It is concluded that UV-endonuclease switches a significant part of potentially mutagenic pyrimidine dimers from the UV-induced error-prone repair pathway to error-free excision repair pathway." @default.
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- W1980102015 date "1975-02-01" @default.
- W1980102015 modified "2023-10-16" @default.
- W1980102015 title "Ultraviolet reactivation and ultraviolet mutagenesis of infectious lambda DNA: Strong inhibition by treatment of DNA in vitro with UV-endonuclease from Micrococcus luteus" @default.
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- W1980102015 doi "https://doi.org/10.1016/0027-5107(75)90074-3" @default.
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