Matches in SemOpenAlex for { <https://semopenalex.org/work/W1980111680> ?p ?o ?g. }
- W1980111680 endingPage "4599" @default.
- W1980111680 startingPage "4585" @default.
- W1980111680 abstract "The assembly of FtsZ plays a central role in construction of the cytokinetic Z-ring that orchestrates bacterial cell division. A naturally occurring naphthoquinone, plumbagin, is known to exhibit antibacterial properties against several types of bacteria. In this study, plumbagin was found to perturb formation of the Z-ring in Bacillus subtilis 168 cells and to cause elongation of these cells without an apparent effect on nucleoid segregation, indicating that it may inhibit FtsZ assembly. Furthermore, it bound to purified B. subtilis FtsZ (BsFtsZ) with a dissociation constant of 20.7 ± 5.6 μM, and inhibited the assembly and GTPase activity of BsFtsZ in vitro. Interestingly, plumbagin did not inhibit either the assembly or GTPase activity of Escherichia coli FtsZ (EcFtsZ) in vitro. Using docking analysis, a putative plumbagin-binding site on BsFtsZ was identified, and the analysis indicated that hydrophobic interactions and hydrogen bonds predominate. Based on the in silico analysis, two variants of BsFtsZ, namely D199A and V307R, were constructed to explore the binding interaction of plumbagin and BsFtsZ. The effects of plumbagin on the assembly and GTPase activity of the variant BsFtsZ proteins in vitro indicated that the residues D199 and V307 may be involved in the binding of plumbagin to BsFtsZ. The results suggest that plumbagin inhibits bacterial proliferation by inhibiting the assembly of FtsZ, and provide insight into the binding site of plumbagin on BsFtsZ, which may help in the design of potent FtsZ-targeted antibacterial agents." @default.
- W1980111680 created "2016-06-24" @default.
- W1980111680 creator A5014454708 @default.
- W1980111680 creator A5026724807 @default.
- W1980111680 creator A5031393032 @default.
- W1980111680 creator A5035863623 @default.
- W1980111680 creator A5056505371 @default.
- W1980111680 date "2013-08-02" @default.
- W1980111680 modified "2023-10-05" @default.
- W1980111680 title "Plumbagin inhibits cytokinesis in<i>Bacillus subtilis</i>by inhibiting FtsZ assembly - a mechanistic study of its antibacterial activity" @default.
- W1980111680 cites W1551931873 @default.
- W1980111680 cites W155286047 @default.
- W1980111680 cites W181618426 @default.
- W1980111680 cites W1819433153 @default.
- W1980111680 cites W1966023025 @default.
- W1980111680 cites W1979973356 @default.
- W1980111680 cites W1986181072 @default.
- W1980111680 cites W1986191025 @default.
- W1980111680 cites W2000203440 @default.
- W1980111680 cites W2001380876 @default.
- W1980111680 cites W2004941701 @default.
- W1980111680 cites W2016612127 @default.
- W1980111680 cites W2022183603 @default.
- W1980111680 cites W2027016017 @default.
- W1980111680 cites W2029181073 @default.
- W1980111680 cites W2030853587 @default.
- W1980111680 cites W2032257802 @default.
- W1980111680 cites W2038589261 @default.
- W1980111680 cites W2039841519 @default.
- W1980111680 cites W2040676542 @default.
- W1980111680 cites W2042975711 @default.
- W1980111680 cites W2044217069 @default.
- W1980111680 cites W2047219204 @default.
- W1980111680 cites W2050000908 @default.
- W1980111680 cites W2051948984 @default.
- W1980111680 cites W2055312279 @default.
- W1980111680 cites W2058541432 @default.
- W1980111680 cites W2064755153 @default.
- W1980111680 cites W2094067130 @default.
- W1980111680 cites W2095172822 @default.
- W1980111680 cites W2099393783 @default.
- W1980111680 cites W2111328511 @default.
- W1980111680 cites W2111620429 @default.
- W1980111680 cites W2114367386 @default.
- W1980111680 cites W2123989172 @default.
- W1980111680 cites W2129995310 @default.
- W1980111680 cites W2130479394 @default.
- W1980111680 cites W2132249028 @default.
- W1980111680 cites W2151801929 @default.
- W1980111680 cites W2153386849 @default.
- W1980111680 cites W2154191173 @default.
- W1980111680 cites W2158534713 @default.
- W1980111680 cites W2159362358 @default.
- W1980111680 cites W2160107613 @default.
- W1980111680 cites W2163233818 @default.
- W1980111680 cites W2168985590 @default.
- W1980111680 cites W2169955182 @default.
- W1980111680 cites W2324573217 @default.
- W1980111680 cites W4293247451 @default.
- W1980111680 doi "https://doi.org/10.1111/febs.12429" @default.
- W1980111680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23841620" @default.
- W1980111680 hasPublicationYear "2013" @default.
- W1980111680 type Work @default.
- W1980111680 sameAs 1980111680 @default.
- W1980111680 citedByCount "47" @default.
- W1980111680 countsByYear W19801116802014 @default.
- W1980111680 countsByYear W19801116802015 @default.
- W1980111680 countsByYear W19801116802016 @default.
- W1980111680 countsByYear W19801116802017 @default.
- W1980111680 countsByYear W19801116802018 @default.
- W1980111680 countsByYear W19801116802019 @default.
- W1980111680 countsByYear W19801116802020 @default.
- W1980111680 countsByYear W19801116802021 @default.
- W1980111680 countsByYear W19801116802022 @default.
- W1980111680 countsByYear W19801116802023 @default.
- W1980111680 crossrefType "journal-article" @default.
- W1980111680 hasAuthorship W1980111680A5014454708 @default.
- W1980111680 hasAuthorship W1980111680A5026724807 @default.
- W1980111680 hasAuthorship W1980111680A5031393032 @default.
- W1980111680 hasAuthorship W1980111680A5035863623 @default.
- W1980111680 hasAuthorship W1980111680A5056505371 @default.
- W1980111680 hasBestOaLocation W19801116801 @default.
- W1980111680 hasConcept C104317684 @default.
- W1980111680 hasConcept C104690221 @default.
- W1980111680 hasConcept C107827726 @default.
- W1980111680 hasConcept C118716 @default.
- W1980111680 hasConcept C1491633281 @default.
- W1980111680 hasConcept C159110408 @default.
- W1980111680 hasConcept C181199279 @default.
- W1980111680 hasConcept C185592680 @default.
- W1980111680 hasConcept C207332259 @default.
- W1980111680 hasConcept C2775905019 @default.
- W1980111680 hasConcept C2776118409 @default.
- W1980111680 hasConcept C2776237524 @default.
- W1980111680 hasConcept C2777272437 @default.
- W1980111680 hasConcept C2780532915 @default.
- W1980111680 hasConcept C29537977 @default.
- W1980111680 hasConcept C41685203 @default.