FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1980462742> ?p ?o ?g. }

• W1980462742 endingPage "8573" @default.
• W1980462742 startingPage "8568" @default.
• W1980462742 abstract "We tested the ability of recombinant hMutSα (hMSH2/hMSH6) and hMutSβ (hMSH2/hMSH3) heterodimers to complement the mismatch repair defect of HEC59, a human cancer cell line whose extracts lack all three MutS homologues. Although repair of both base/base mispairs and insertion–deletion loops was restored by hMutSα, only the latter substrates were addressed in extracts supplemented with hMutSβ. hMutSα was also able to complement a defect in the repair of base/base mispairs in CHO R and HL60R cell extracts. In these cells, methotrexate-induced amplification of the dihydrofolate reductase ( DHFR ) locus, which also contains the MSH3 gene, led to an overexpression of MSH3 and thus to a dramatic change in the relative levels of MutSα and MutSβ. As a rule, MSH2 is primarily complexed with MSH6. MutSα is thus relatively abundant in mammalian cell extracts, whereas MutSβ levels are generally low. In contrast, in cells that overexpress MSH3, the available MSH2 protein is sequestered predominantly into MutSβ. This leads to degradation of the partnerless MSH6 and depletion of MutSα. CHO R and HL60R cells therefore lack correction of base/base mispairs, whereas loop repair is maintained by MutSβ. Consequently, frameshift mutations in CHO R are rare, whereas transitions and transversions are acquired at a rate two orders of magnitude above background. Our data thus support and extend the findings of Drummond et al. [Drummond, J. T., Genschel, J., Wolf, E. & Modrich, P. (1997) Proc. Natl. Acad. Sci. USA 94, 10144–10149] and demonstrate that mismatch repair deficiency can arise not only through mutation or transcriptional silencing of a mismatch repair gene, but also as a result of imbalance in the relative amounts of the MSH3 and MSH6 proteins." @default.
• W1980462742 created "2016-06-24" @default.
• W1980462742 creator A5003267441 @default.
• W1980462742 creator A5006024704 @default.
• W1980462742 creator A5015058883 @default.
• W1980462742 creator A5023899260 @default.
• W1980462742 creator A5066096686 @default.
• W1980462742 creator A5071043734 @default.
• W1980462742 date "1998-07-21" @default.
• W1980462742 modified "2023-10-13" @default.
• W1980462742 title "Mismatch repair deficiency associated with overexpression of the <i>MSH3</i> gene" @default.
• W1980462742 cites W1494559876 @default.
• W1980462742 cites W1496112877 @default.
• W1980462742 cites W1506709601 @default.
• W1980462742 cites W1569389754 @default.
• W1980462742 cites W1593208137 @default.
• W1980462742 cites W1593968937 @default.
• W1980462742 cites W1600706591 @default.
• W1980462742 cites W1783347833 @default.
• W1980462742 cites W1793411864 @default.
• W1980462742 cites W1848156229 @default.
• W1980462742 cites W1967205646 @default.
• W1980462742 cites W1971715978 @default.
• W1980462742 cites W1977284533 @default.
• W1980462742 cites W1983811214 @default.
• W1980462742 cites W2007973565 @default.
• W1980462742 cites W2012732208 @default.
• W1980462742 cites W2012867725 @default.
• W1980462742 cites W2016723153 @default.
• W1980462742 cites W2017363371 @default.
• W1980462742 cites W2019932549 @default.
• W1980462742 cites W2055993339 @default.
• W1980462742 cites W2064700784 @default.
• W1980462742 cites W2075437346 @default.
• W1980462742 cites W2076897901 @default.
• W1980462742 cites W2085896453 @default.
• W1980462742 cites W2094983671 @default.
• W1980462742 cites W2137334007 @default.
• W1980462742 cites W2139964075 @default.
• W1980462742 cites W2147555368 @default.
• W1980462742 cites W2157081670 @default.
• W1980462742 cites W2170472485 @default.
• W1980462742 cites W2292680730 @default.
• W1980462742 cites W2415662541 @default.
• W1980462742 cites W2464005404 @default.
• W1980462742 cites W4230746983 @default.
• W1980462742 doi "https://doi.org/10.1073/pnas.95.15.8568" @default.
• W1980462742 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/21116" @default.
• W1980462742 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9671718" @default.
• W1980462742 hasPublicationYear "1998" @default.
• W1980462742 type Work @default.
• W1980462742 sameAs 1980462742 @default.
• W1980462742 citedByCount "202" @default.
• W1980462742 countsByYear W19804627422012 @default.
• W1980462742 countsByYear W19804627422013 @default.
• W1980462742 countsByYear W19804627422014 @default.
• W1980462742 countsByYear W19804627422015 @default.
• W1980462742 countsByYear W19804627422016 @default.
• W1980462742 countsByYear W19804627422017 @default.
• W1980462742 countsByYear W19804627422018 @default.
• W1980462742 countsByYear W19804627422019 @default.
• W1980462742 countsByYear W19804627422020 @default.
• W1980462742 countsByYear W19804627422021 @default.
• W1980462742 countsByYear W19804627422022 @default.
• W1980462742 countsByYear W19804627422023 @default.
• W1980462742 crossrefType "journal-article" @default.
• W1980462742 hasAuthorship W1980462742A5003267441 @default.
• W1980462742 hasAuthorship W1980462742A5006024704 @default.
• W1980462742 hasAuthorship W1980462742A5015058883 @default.
• W1980462742 hasAuthorship W1980462742A5023899260 @default.
• W1980462742 hasAuthorship W1980462742A5066096686 @default.
• W1980462742 hasAuthorship W1980462742A5071043734 @default.
• W1980462742 hasBestOaLocation W19804627422 @default.
• W1980462742 hasConcept C104317684 @default.
• W1980462742 hasConcept C134935766 @default.
• W1980462742 hasConcept C153911025 @default.
• W1980462742 hasConcept C185581394 @default.
• W1980462742 hasConcept C187206112 @default.
• W1980462742 hasConcept C2776674815 @default.
• W1980462742 hasConcept C2779115348 @default.
• W1980462742 hasConcept C29906990 @default.
• W1980462742 hasConcept C501734568 @default.
• W1980462742 hasConcept C54355233 @default.
• W1980462742 hasConcept C552990157 @default.
• W1980462742 hasConcept C60748783 @default.
• W1980462742 hasConcept C86803240 @default.
• W1980462742 hasConceptScore W1980462742C104317684 @default.
• W1980462742 hasConceptScore W1980462742C134935766 @default.
• W1980462742 hasConceptScore W1980462742C153911025 @default.
• W1980462742 hasConceptScore W1980462742C185581394 @default.
• W1980462742 hasConceptScore W1980462742C187206112 @default.
• W1980462742 hasConceptScore W1980462742C2776674815 @default.
• W1980462742 hasConceptScore W1980462742C2779115348 @default.
• W1980462742 hasConceptScore W1980462742C29906990 @default.
• W1980462742 hasConceptScore W1980462742C501734568 @default.
• W1980462742 hasConceptScore W1980462742C54355233 @default.
• W1980462742 hasConceptScore W1980462742C552990157 @default.
• W1980462742 hasConceptScore W1980462742C60748783 @default.

• next