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- W1980469208 abstract "A computerized system which greatly accelerates and eases the collection, storage, and analysis of data has been applied to several standard biochemical assays. The system uses a commercially available microtiter plate reader connected to an Apple IIe microcomputer via a standard serial port. Data are transmitted automatically from the reader to the microcomputer, where they can be viewed, printed, further analyzed immediately, or stored on a diskette for later retrieval and processing. Some or all of the data may be entered manually. The program calculates a linear least squares best fit to a standard curve after correcting all data for blanks, then determines the quantities of substrate or product contained in each well of a microtiter plate. Data from two plates may be combined, enabling calculation of enzyme specific activities. This system can be adapted to any assay whose final step can be performed by a microtiter plate reader. Its use is described for determination of protein concentration, Na,K-ATPase activity, and K-stimulated p-nitrophenylphosphatase activity." @default.
- W1980469208 created "2016-06-24" @default.
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- W1980469208 date "1986-05-01" @default.
- W1980469208 modified "2023-09-27" @default.
- W1980469208 title "A rapid, flexibel method for biochemical assays using a microtiter plate reader and a microcomputer. Application for assays of protein, Na,K-Atpase and K-p-Nitrophenylphosphatase" @default.
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- W1980469208 doi "https://doi.org/10.1016/0020-7101(86)90015-2" @default.
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