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- W1980564071 abstract "Bovine retinas were incubated with 3RS-[5-3H]-mevalonic acid under conditions similar to those previously shown to support opsin biosynthesis in vitro. TLC of the total lipids indicated the formation of numerous radiolabeled components, including sterols, hydrocarbons, and fatty acid-like material. The nonsaponifiable lipids were analyzed by TLC, GLC, and chromatography on columns of silicic acid-Super Cel, silica gel G-Super Cel-silver nitrate, and alumina-Super Cel-silver nitrate. The major nonsaponifiable components had the chromatographic properties of squalene and methylated sterols (i.e., C30, C29, and C28 monohydroxy sterols). Cholesterol represented no more than 1% of the total radioactivity in the nonsaponifiable lipid fraction. The fatty acid-like material was derivatized with diazomethane, and the resulting methyl esters were analyzed by GLC before and after catalytic hydrogenation. The radioactivity did not correspond to the normal fatty acids endogenous to the retina, but rather had the chromatographic properties of C15 and C20 isoprenoid acids. These results obtained with intact retinas are consistent with our previous observations concerning mevalonic acid metabolism in cell-free homogenates of bovine retinas." @default.
- W1980564071 created "2016-06-24" @default.
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- W1980564071 date "1986-02-01" @default.
- W1980564071 modified "2023-09-26" @default.
- W1980564071 title "In Vitro Metabolism of Mevalonic Acid in the Bovine Retina" @default.
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- W1980564071 doi "https://doi.org/10.1111/j.1471-4159.1986.tb12989.x" @default.
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