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• W1980665017 abstract "Abstract 1. The chemical analysis and CsCl buoyant density gradient centrifugation showed that the protein content of the 32-S ribosomal subunit, prepared from rat liver ribosomes by EDTA treatment, was lower than that of the active 40-S subunit, prepared by incubation with a high concentration of KCl. The same analyses of the EDTA-treated 47-S subunit and the active 60-S subunit showed similar protein contents between two types of ribosomal large subunits. The EDTA-treated 32-S subunit prepared in the presence of the liver ribonuclease inhibitor showed poly(U)-binding activity comparable to the active 40-S subunit, although the activity of poly(U)-dependent polyphenylalanine synthesis was very low. 2. Protein-depleted particles (40-S core particles) and split proteins were prepared from the active 40-S subunit by treatment with 0.4 M LiCl in the presence of 5 mM MgCl 2 . About 25 % of protein was dissociated from the active 40-S subunit by this treatment. The selective dissociation of proteins from 40-S subunit was shown by acrylamide gel electrophoresis of split proteins and 40-S core particles. The reconstructed particles from core particles and split proteins from the active 40-S subunit showed definite activity in poly(U)-dependent polyphenylalanine synthesis in conjunction with the active 60-S ribosomal subunit. 3. By incubation of 18-S ribosomal RNA with proteins of the 40-S ribosomal subunit, particles with s value of about 32 S were reconstructed. The protein content of the 32-S reconstructed particles was somewhat lower than that of the EDTA-treated 32-S subunit and acrylamide gel electrophoresis of the proteins of the particles showed a complete set of protein components of the original 40-S subunit, although the densitogram showed a difference in some bands between the 40-S subunit and the reconstructed particles. The reconstructed 32-S particles showed definite activity in poly(U)-binding, but their activity in poly(U)-dependent poly-phenylalanine synthesis in conjunction with the active 60-S sbunit was very low." @default.
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• W1980665017 date "1971-12-01" @default.
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• W1980665017 title "Studies on the small subunit of rat liver ribosomes: Some biochemical properties with specific references to the reconstruction of the small subunit" @default.
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• W1980665017 doi "https://doi.org/10.1016/0005-2787(71)90837-9" @default.
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