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- W1980692308 abstract "ABSTRACTNanopore-based sensor is a new single-molecule level sensing method, which involves detecting the currentchange and translocation time of the molecule when it passes through the pore by the electrophoresis. In thiswork, the lambda-DNA was driven thread through the solid-state nanopore of different diameters to characterizeDNA’s physical properties from its translocation process in 1 M KCl salt solution. Firstly, the frequency of eventsincreased with the rising voltages. We found that the event frequency for 200 mV decreased because of thereduced concentration of the -DNA after about two hours’ translocation process under other voltages. Andthe duration times of lambda-DNA translocation events which linearly passed through the nanopore at differentvoltages were analyzed with the “rst-passage-time distribution indicated that the electrophoretic mobility oflambda-DNA is 1.153× 10 10 m 2 V 1 s 1 under the condition of 1 M KCl buffer and 22 nm nanopore. Besides,we investigated two methods to calculate the diffusion constant D. The two results agreed with each otherquite well. The capture rate increased with the voltage at a “xed DNA concentration. The data analysis of DNApassing through nanopores with different sizes suggested that the diameter of the nanopore plays a key factorin DNA translocation behavior. This is an important result for the label-free sensing by solid-state nanopore inthe future and will provide more evidences for the development of nanopores as a multi-functional sensor for awide range of biopolymers and nanoparticles.KEYWORDS: Solid-State Nanopore, Double-Strand DNA, Translocation Time, Current Blockage,Electrophoretic Mobility, Diffusion Constant." @default.
- W1980692308 created "2016-06-24" @default.
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- W1980692308 date "2013-12-01" @default.
- W1980692308 modified "2023-09-23" @default.
- W1980692308 title "Voltage Dependence Resistive Pulse of Lambda-DNA Translocation with Different Size Solid-State Nanopore Sensor" @default.
- W1980692308 cites W2132905138 @default.
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- W1980692308 doi "https://doi.org/10.1166/sam.2013.1704" @default.
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