Matches in SemOpenAlex for { <https://semopenalex.org/work/W1980898678> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W1980898678 endingPage "20" @default.
- W1980898678 startingPage "20" @default.
- W1980898678 abstract "The centromere-kinetochore complex is a chromosomal assembly site including repeat motifs and protein binding properties thus mediating chromosome motility and mitotic regulation. Next to the α-satellite DNA family as well as human satellite III DNA, contribution of other repetitive sequences has to be strongly considered in centromere function. Here, we report the identification of centromeric 48 bp motifs, isolated from chimpanzee and orang-utan using an orthologous human DNA probe. Applying Southern hybridization we show that these sequences are restricted to hominoid species. Diminishing hybrid formation in gibbons suggested that the 48 bp repeat originated 25–20 million years ago. Consistently, both chimpanzee as well as human repeat probes failed to generate any hybridization signal with the monkey species Cercopithecus aethiops and Aotes trivirgatus. Sequence deviations from the consensus of human repeat monomers of 6% and 10.4% in chimpanzee and orang-utan, respectively, were found within a 16 bp region of the 48 bp repeat units. Gel mobility shift assays using chimpanzee repeat dimers as probes revealed peptide-binding properties with human and chimpanzee nuclear extracts. Species-specific DNA-protein complexes remained unaffected by competition studies and indicated the presence of at least one novel interacting protein consisting of two subunits with 90 and 95 kDa. Our data suggest that the 48 bp repeat, next to α-satellite DNA, provides essential sequence information for specific DNA-protein interaction and they imply phylogenetic conservation of these binding properties in primates. The complex is likely involved in the proper formation and/or function of mammalian centromeres." @default.
- W1980898678 created "2016-06-24" @default.
- W1980898678 creator A5000816192 @default.
- W1980898678 creator A5021424767 @default.
- W1980898678 creator A5029397310 @default.
- W1980898678 creator A5058466381 @default.
- W1980898678 creator A5079519045 @default.
- W1980898678 date "2002-01-01" @default.
- W1980898678 modified "2023-09-27" @default.
- W1980898678 title "The 48 bp centromeric repeat is a functionally conserved motif in great apes and man showing protein-binding properties" @default.
- W1980898678 doi "https://doi.org/10.1002/1522-2683(200201)23:1<20::aid-elps20>3.0.co;2-c" @default.
- W1980898678 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11824617" @default.
- W1980898678 hasPublicationYear "2002" @default.
- W1980898678 type Work @default.
- W1980898678 sameAs 1980898678 @default.
- W1980898678 citedByCount "0" @default.
- W1980898678 crossrefType "journal-article" @default.
- W1980898678 hasAuthorship W1980898678A5000816192 @default.
- W1980898678 hasAuthorship W1980898678A5021424767 @default.
- W1980898678 hasAuthorship W1980898678A5029397310 @default.
- W1980898678 hasAuthorship W1980898678A5058466381 @default.
- W1980898678 hasAuthorship W1980898678A5079519045 @default.
- W1980898678 hasConcept C104317684 @default.
- W1980898678 hasConcept C140367098 @default.
- W1980898678 hasConcept C141231307 @default.
- W1980898678 hasConcept C153911025 @default.
- W1980898678 hasConcept C169802333 @default.
- W1980898678 hasConcept C193252679 @default.
- W1980898678 hasConcept C199216141 @default.
- W1980898678 hasConcept C3017666073 @default.
- W1980898678 hasConcept C30481170 @default.
- W1980898678 hasConcept C54355233 @default.
- W1980898678 hasConcept C552990157 @default.
- W1980898678 hasConcept C63491729 @default.
- W1980898678 hasConcept C78458016 @default.
- W1980898678 hasConcept C86803240 @default.
- W1980898678 hasConceptScore W1980898678C104317684 @default.
- W1980898678 hasConceptScore W1980898678C140367098 @default.
- W1980898678 hasConceptScore W1980898678C141231307 @default.
- W1980898678 hasConceptScore W1980898678C153911025 @default.
- W1980898678 hasConceptScore W1980898678C169802333 @default.
- W1980898678 hasConceptScore W1980898678C193252679 @default.
- W1980898678 hasConceptScore W1980898678C199216141 @default.
- W1980898678 hasConceptScore W1980898678C3017666073 @default.
- W1980898678 hasConceptScore W1980898678C30481170 @default.
- W1980898678 hasConceptScore W1980898678C54355233 @default.
- W1980898678 hasConceptScore W1980898678C552990157 @default.
- W1980898678 hasConceptScore W1980898678C63491729 @default.
- W1980898678 hasConceptScore W1980898678C78458016 @default.
- W1980898678 hasConceptScore W1980898678C86803240 @default.
- W1980898678 hasIssue "1" @default.
- W1980898678 hasLocation W19808986781 @default.
- W1980898678 hasLocation W19808986782 @default.
- W1980898678 hasOpenAccess W1980898678 @default.
- W1980898678 hasPrimaryLocation W19808986781 @default.
- W1980898678 hasRelatedWork W1966013362 @default.
- W1980898678 hasRelatedWork W1989675374 @default.
- W1980898678 hasRelatedWork W2013725336 @default.
- W1980898678 hasRelatedWork W2027536051 @default.
- W1980898678 hasRelatedWork W2087241912 @default.
- W1980898678 hasRelatedWork W2145335676 @default.
- W1980898678 hasRelatedWork W2378224211 @default.
- W1980898678 hasRelatedWork W4307497505 @default.
- W1980898678 hasRelatedWork W4360942832 @default.
- W1980898678 hasRelatedWork W2519500480 @default.
- W1980898678 hasVolume "23" @default.
- W1980898678 isParatext "false" @default.
- W1980898678 isRetracted "false" @default.
- W1980898678 magId "1980898678" @default.
- W1980898678 workType "article" @default.