FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1980913885> ?p ?o ?g. }

• W1980913885 endingPage "15647" @default.
• W1980913885 startingPage "15635" @default.
• W1980913885 abstract "The RNA-binding proteins TDP-43 and FUS form abnormal cytoplasmic aggregates in affected tissues of patients with amyotrophic lateral sclerosis and frontotemporal lobar dementia. TDP-43 and FUS localize mainly in the nucleus where they regulate pre-mRNA splicing, but they are also involved in mRNA transport, stability, and translation. To better investigate their cytoplasmic activities, we applied an RNA immunoprecipitation and chip analysis to define the mRNAs associated to TDP-43 and FUS in the cytoplasmic ribonucleoprotein complexes from motoneuronal NSC-34 cells. We found that they bind different sets of mRNAs although converging on common cellular pathways. Bioinformatics analyses identified the (UG)(n) consensus motif in 80% of 3'-UTR sequences of TDP-43 targets, whereas for FUS the binding motif was less evident. By in vitro assays we validated binding to selected target 3'-UTRs, including Vegfa and Grn for TDP-43, and Vps54, Nvl, and Taf15 for FUS. We showed that TDP-43 has a destabilizing activity on Vegfa and Grn mRNAs and may ultimately affect progranulin protein content, whereas FUS does not affect mRNA stability/translation of its targets. We also demonstrated that three different point mutations in TDP-43 did not change the binding affinity for Vegfa and Grn mRNAs or their protein level. Our data indicate that TDP-43 and FUS recognize distinct sets of mRNAs and differently regulate their fate in the cytoplasm of motoneuron-like cells, therefore suggesting complementary roles in neuronal RNA metabolism and neurodegeneration." @default.
• W1980913885 created "2016-06-24" @default.
• W1980913885 creator A5000774656 @default.
• W1980913885 creator A5021084166 @default.
• W1980913885 creator A5025840282 @default.
• W1980913885 creator A5029821144 @default.
• W1980913885 creator A5032436476 @default.
• W1980913885 creator A5033472971 @default.
• W1980913885 creator A5048517130 @default.
• W1980913885 creator A5086706420 @default.
• W1980913885 date "2012-05-01" @default.
• W1980913885 modified "2023-10-12" @default.
• W1980913885 title "TDP-43 and FUS RNA-binding Proteins Bind Distinct Sets of Cytoplasmic Messenger RNAs and Differently Regulate Their Post-transcriptional Fate in Motoneuron-like Cells" @default.
• W1980913885 cites W1525431743 @default.
• W1980913885 cites W1619082318 @default.
• W1980913885 cites W1966364136 @default.
• W1980913885 cites W1967443709 @default.
• W1980913885 cites W1969196565 @default.
• W1980913885 cites W1981727471 @default.
• W1980913885 cites W1983335981 @default.
• W1980913885 cites W1984145170 @default.
• W1980913885 cites W1988097332 @default.
• W1980913885 cites W1993556679 @default.
• W1980913885 cites W1995550072 @default.
• W1980913885 cites W1995635963 @default.
• W1980913885 cites W1995715453 @default.
• W1980913885 cites W2002506816 @default.
• W1980913885 cites W2003618511 @default.
• W1980913885 cites W2005731595 @default.
• W1980913885 cites W2006797111 @default.
• W1980913885 cites W2010659088 @default.
• W1980913885 cites W2011184139 @default.
• W1980913885 cites W2012218517 @default.
• W1980913885 cites W2016332933 @default.
• W1980913885 cites W2018017154 @default.
• W1980913885 cites W2023166679 @default.
• W1980913885 cites W2027460468 @default.
• W1980913885 cites W2030411520 @default.
• W1980913885 cites W2031964319 @default.
• W1980913885 cites W2032719480 @default.
• W1980913885 cites W2036379239 @default.
• W1980913885 cites W2038438413 @default.
• W1980913885 cites W2046407150 @default.
• W1980913885 cites W2048813950 @default.
• W1980913885 cites W2049342904 @default.
• W1980913885 cites W2051470096 @default.
• W1980913885 cites W2052818103 @default.
• W1980913885 cites W2053272214 @default.
• W1980913885 cites W2054274682 @default.
• W1980913885 cites W2060706160 @default.
• W1980913885 cites W2066952414 @default.
• W1980913885 cites W2066972125 @default.
• W1980913885 cites W2073424121 @default.
• W1980913885 cites W2075186362 @default.
• W1980913885 cites W2075588816 @default.
• W1980913885 cites W2078061248 @default.
• W1980913885 cites W2079470515 @default.
• W1980913885 cites W2085717785 @default.
• W1980913885 cites W2086896046 @default.
• W1980913885 cites W2088017477 @default.
• W1980913885 cites W2101042581 @default.
• W1980913885 cites W2108191354 @default.
• W1980913885 cites W2112523914 @default.
• W1980913885 cites W2113524952 @default.
• W1980913885 cites W2117428493 @default.
• W1980913885 cites W2121868653 @default.
• W1980913885 cites W2125948889 @default.
• W1980913885 cites W2126569879 @default.
• W1980913885 cites W2130828395 @default.
• W1980913885 cites W2135457495 @default.
• W1980913885 cites W2136448211 @default.
• W1980913885 cites W2136949432 @default.
• W1980913885 cites W2141231623 @default.
• W1980913885 cites W2145091349 @default.
• W1980913885 cites W2150057773 @default.
• W1980913885 cites W2154417593 @default.
• W1980913885 cites W2156512271 @default.
• W1980913885 cites W2157952888 @default.
• W1980913885 cites W2170089448 @default.
• W1980913885 doi "https://doi.org/10.1074/jbc.m111.333450" @default.
• W1980913885 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3346140" @default.
• W1980913885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22427648" @default.
• W1980913885 hasPublicationYear "2012" @default.
• W1980913885 type Work @default.
• W1980913885 sameAs 1980913885 @default.
• W1980913885 citedByCount "231" @default.
• W1980913885 countsByYear W19809138852012 @default.
• W1980913885 countsByYear W19809138852013 @default.
• W1980913885 countsByYear W19809138852014 @default.
• W1980913885 countsByYear W19809138852015 @default.
• W1980913885 countsByYear W19809138852016 @default.
• W1980913885 countsByYear W19809138852017 @default.
• W1980913885 countsByYear W19809138852018 @default.
• W1980913885 countsByYear W19809138852019 @default.
• W1980913885 countsByYear W19809138852020 @default.
• W1980913885 countsByYear W19809138852021 @default.
• W1980913885 countsByYear W19809138852022 @default.
• W1980913885 countsByYear W19809138852023 @default.

• next