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• W1980921751 abstract "We have determined the complete structural arrangement of LAMA4, the gene encoding the laminin α4 chain. Using both yeast artificial chromosome clones and total human genomic DNA and primers derived from the cDNA sequence, regions of the gene were amplified and sequenced to determine the splice donor and acceptor sites. The introns were sized by agarose gel electrophoresis of the PCR products. The gene consisted of 39 exons spanning 122 kb. All of the splice sites conformed to the GTIAG rule, except intron 7 which possessed a GC dinucleotide at the donor splice site. The introdexon ratio was large at 17.8:1, mainly due to large introns at the 5' end of the gene. Regions at both the 5' and 3' end of the gene were subcloned from the yeast artificial chromosomes to enable untranscribed DNA to be sequenced. The gene represents the second of the laminin A gene family to be characterised and its structural organisation is similar to the equivalent regions of the LAMA2 gene." @default.
• W1980921751 created "2016-06-24" @default.
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• W1980921751 date "1997-08-15" @default.
• W1980921751 modified "2023-10-14" @default.
• W1980921751 title "The Structural Organisation of LAMA4, the Gene Encoding Laminin alphaL4" @default.
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• W1980921751 doi "https://doi.org/10.1111/j.1432-1033.1997.t01-1-00015.x" @default.
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