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- W1980999742 abstract "Indirect immunofluorescent assay for antinuclear antibodies (IIFA-ANA) on HEp-2 cell substrate is a widely used test for diagnosis of connective tissue diseases. Recently, serum-free fibroblast cell line McCoy-Plovdiv has been developed to provide an alternative substrate. The aim of the present study was to evaluate the diagnostic performance of IIFA-ANA on McCoy-Plovdiv cell substrate for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and compare it with that of the standard HEp-2 cell substrate. Sera from 72 patients with RA and 23 patients with SLE were tested with IIFA-ANA on both substrates. The control group consisted of 100 sera from healthy individuals. The agreement (Cohen's κ) and the diagnostic performance (ROC analysis) of both methods were evaluated. IIFA-ANA on McCoy-Plovdiv cells proved to be comparable with the standard IIFA-ANA on HEp-2 cell substrate. Le test d’immunofluorescence indirecte pour détecter les anticorps antinucléaires (IFI-AAN) sur substrat cellulaire HEp-2 est une méthode générale permettant de diagnostiquer les connectivites. La lignée cellulaire sans-sérum, McCoy-Plovdiv peut servir comme substrat alternatif. Le but de cette étude était d’analyser les possibilités diagnostiques d’IFI-AAN pour le lupus érythémateux systémique (LES) et la polyarthrite rhumatoïde (PR) sur le substrat cellulaire McCoy-Plovdiv et de faire des comparaisons avec les résultats obtenus sur le substrat standard HEp-2. Des sérums de 72 patients atteints de polyarthrite rhumatoïde et de 23 patients atteints de lupus érythémateux systémique ont été analysés par le test d’immunofluorescence indirecte sur les deux substrats. Le groupe témoin était constitué de 100 sérums d’individus sains. On a constaté un degré de coïncidence entre les deux méthodes (coefficient kappa de Cohen) et on a comparé leurs possibilités diagnostiques (analyse-ROC). Le test d’immunofluorescence indirecte pour la recherche des anticorps antinucléaires a donné des résultats équivalents sur les deux substrats." @default.
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- W1980999742 date "2007-05-01" @default.
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- W1980999742 title "Indirect immunofluorescent assay for antinuclear antibodies on McCoy-Plovdiv serum-free cell line substrate" @default.
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- W1980999742 doi "https://doi.org/10.1016/j.cimid.2006.11.007" @default.
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