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• W1981013488 abstract "Protein tyrosine nitration is a post-translational modification (PTM) mediated by nitric oxide-derived molecules. Peroxisomes are oxidative organelles in which the presence of nitric oxide (NO) has been reported. We studied peroxisomal nitroproteome of pea leaves by high-performance liquid chromatography with tandem mass spectrometry (LC–MS/MS) and proteomic approaches. Proteomic analysis of peroxisomes from pea leaves detected a total of four nitro-tyrosine immunopositive proteins by using an antibody against nitrotyrosine. One of these proteins was found to be the NADH-dependent hydroxypyruvate reductase (HPR). The in vitro nitration of peroxisomal samples caused a 65% inhibition of HPR activity. Analysis of recombinant peroxisomal NADH-dependent HPR1 activity from Arabidopsis in the presence of H2O2, NO, GSH and peroxynitrite showed that the ONOO− molecule caused the highest inhibition of activity (51% at 5 mM SIN-1), with 5 mM H2O2 having no inhibitory effect. Mass spectrometric analysis of the nitrated recombinant HPR1 enabled us to determine that, among the eleven tyrosine present in this enzyme, only Tyr-97, Tyr-108 and Tyr-198 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Site-directed mutagenesis confirmed Tyr198 as the primary site of nitration responsible for the inhibition on the enzymatic activity by peroxynitrite. These findings suggest that peroxisomal HPR is a target of peroxynitrite which provokes a loss of function. This is the first report demonstrating the peroxisomal NADH-dependent HPR activity involved in the photorespiration pathway is regulated by tyrosine nitration, indicating that peroxisomal NO metabolism may contribute to the regulation of physiological processes under no-stress conditions." @default.
• W1981013488 created "2016-06-24" @default.
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• W1981013488 date "2013-11-01" @default.
• W1981013488 modified "2023-10-18" @default.
• W1981013488 title "Inhibition of peroxisomal hydroxypyruvate reductase (HPR1) by tyrosine nitration" @default.
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• W1981013488 cites W1968022393 @default.
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• W1981013488 cites W1989228404 @default.
• W1981013488 cites W1990532535 @default.
• W1981013488 cites W1991235962 @default.
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• W1981013488 cites W2012352784 @default.
• W1981013488 cites W2015360357 @default.
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• W1981013488 cites W2019695253 @default.
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• W1981013488 cites W2035326812 @default.
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• W1981013488 cites W2062275190 @default.
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• W1981013488 doi "https://doi.org/10.1016/j.bbagen.2013.07.002" @default.
• W1981013488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23860243" @default.
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