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- W1981016739 abstract "Membranes prepared from Rhodopseudomonas capsulata grown heterotrophically in the dark perform phosphorylation linked to oxidation of NADH and succinate, with P2e− ratios of about 0.5 and 0.15, respectively. The localization of the sites of energy conservation was investigated by observing the respiration-induced quenching of the fluorescence of atebrine. Energization of the membrane can be demonstrated when NADH is oxidized by O2, ferricyanide or Q1, when succinate is oxidized by O2 or by oxidized diaminodurene, and during the oxidation of reduced diaminodurene. Antimycin A completely inhibits energization between succinate and O2 or succinate and diaminodurene; however, it only inhibits partially NADH or succinate oxidases and energization between NADH and O2. KCN inhibits NADH oxidase in a biphasic way: the first level of inhibition is observed at concentrations which block the oxidation of exogenous cytochrome c or of diaminodurene and energization between succinate or ascorbate-diaminodurene and O2. The second level corresponds to the inhibition of the antimycin-insensitive oxidase. The results are interpreted as evidence of the presence in these bacteria of a respiratory chain branching after the dehydrogenase system, one arm of the chain being sensitive to antimycin A and low concentrations of KCN and capable of energy conservation, the other being represented by a completely uncoupled system." @default.
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- W1981016739 date "1973-09-01" @default.
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- W1981016739 title "Energy transduction in photosynthetic bacteria VI. Respiratory sites of energy conservation in membranes from dark-grown cells of Rhodopseudomonas capsulata" @default.
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- W1981016739 doi "https://doi.org/10.1016/0005-2728(73)90114-x" @default.
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