Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981018711> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1981018711 endingPage "68" @default.
- W1981018711 startingPage "63" @default.
- W1981018711 abstract "Human CYP3A4, the major human, intestinal, drug metabolizing cytochrome P450, has been introduced into three mammalian cell lines (Caco-2, MDCK and LLC-PK1) suitable for making drug permeability measurements. The levels and stability of expression were analyzed by enzyme assays (testosterone 6β-hydroxylase and nifedipine oxidase). Long term, stable CYP3A4 expression/cell growth rate was obtained in MDCK cells. In the LLC-PK1 system, shorter term, stable expression was achieved. However, in Caco-2 cells, derivatives with better properties than those previously reported could not be obtained. The highest level of CYP3A4 catalytic activity was obtained in LLC-PK1 cells. In this system, CYP3A4 activity levels appeared comparable to median level human intestinal microsomes. Metabolite formation and inhibition kinetics were examined in cell monolayers. Nifedipine was found to be extensively metabolized (19%) during passage across cell monolayers. In general, affinity related parameters (apparent Km and apparent Ki) were 1.5- to three-fold higher under conditions of flux through the monolayers relative to steady-state conditions. These systems should be useful for examining the role of intestinal CYP3A4 in first-pass metabolism and drug–drug interactions." @default.
- W1981018711 created "2016-06-24" @default.
- W1981018711 creator A5026467025 @default.
- W1981018711 creator A5061295760 @default.
- W1981018711 creator A5072605004 @default.
- W1981018711 creator A5077413863 @default.
- W1981018711 creator A5088332392 @default.
- W1981018711 date "2000-11-01" @default.
- W1981018711 modified "2023-10-18" @default.
- W1981018711 title "Analysis of drug transport and metabolism in cell monolayer systems that have been modified by cytochrome P4503A4 cDNA-expression" @default.
- W1981018711 cites W1520965071 @default.
- W1981018711 cites W1633747844 @default.
- W1981018711 cites W1904678284 @default.
- W1981018711 cites W1978224396 @default.
- W1981018711 cites W1979196526 @default.
- W1981018711 cites W1979732900 @default.
- W1981018711 cites W2015221134 @default.
- W1981018711 cites W201548973 @default.
- W1981018711 cites W2024587809 @default.
- W1981018711 cites W2046827775 @default.
- W1981018711 cites W2073965718 @default.
- W1981018711 cites W2136564762 @default.
- W1981018711 cites W2155020314 @default.
- W1981018711 cites W2168959672 @default.
- W1981018711 cites W323918011 @default.
- W1981018711 doi "https://doi.org/10.1016/s0928-0987(00)00141-x" @default.
- W1981018711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11121734" @default.
- W1981018711 hasPublicationYear "2000" @default.
- W1981018711 type Work @default.
- W1981018711 sameAs 1981018711 @default.
- W1981018711 citedByCount "28" @default.
- W1981018711 countsByYear W19810187112012 @default.
- W1981018711 countsByYear W19810187112013 @default.
- W1981018711 countsByYear W19810187112014 @default.
- W1981018711 countsByYear W19810187112015 @default.
- W1981018711 countsByYear W19810187112018 @default.
- W1981018711 crossrefType "journal-article" @default.
- W1981018711 hasAuthorship W1981018711A5026467025 @default.
- W1981018711 hasAuthorship W1981018711A5061295760 @default.
- W1981018711 hasAuthorship W1981018711A5072605004 @default.
- W1981018711 hasAuthorship W1981018711A5077413863 @default.
- W1981018711 hasAuthorship W1981018711A5088332392 @default.
- W1981018711 hasConcept C109650736 @default.
- W1981018711 hasConcept C1491633281 @default.
- W1981018711 hasConcept C181199279 @default.
- W1981018711 hasConcept C185592680 @default.
- W1981018711 hasConcept C193176779 @default.
- W1981018711 hasConcept C2777477808 @default.
- W1981018711 hasConcept C41183919 @default.
- W1981018711 hasConcept C526171541 @default.
- W1981018711 hasConcept C54355233 @default.
- W1981018711 hasConcept C55493867 @default.
- W1981018711 hasConcept C56856141 @default.
- W1981018711 hasConcept C62231903 @default.
- W1981018711 hasConcept C81885089 @default.
- W1981018711 hasConcept C86803240 @default.
- W1981018711 hasConcept C87644729 @default.
- W1981018711 hasConcept C89311334 @default.
- W1981018711 hasConceptScore W1981018711C109650736 @default.
- W1981018711 hasConceptScore W1981018711C1491633281 @default.
- W1981018711 hasConceptScore W1981018711C181199279 @default.
- W1981018711 hasConceptScore W1981018711C185592680 @default.
- W1981018711 hasConceptScore W1981018711C193176779 @default.
- W1981018711 hasConceptScore W1981018711C2777477808 @default.
- W1981018711 hasConceptScore W1981018711C41183919 @default.
- W1981018711 hasConceptScore W1981018711C526171541 @default.
- W1981018711 hasConceptScore W1981018711C54355233 @default.
- W1981018711 hasConceptScore W1981018711C55493867 @default.
- W1981018711 hasConceptScore W1981018711C56856141 @default.
- W1981018711 hasConceptScore W1981018711C62231903 @default.
- W1981018711 hasConceptScore W1981018711C81885089 @default.
- W1981018711 hasConceptScore W1981018711C86803240 @default.
- W1981018711 hasConceptScore W1981018711C87644729 @default.
- W1981018711 hasConceptScore W1981018711C89311334 @default.
- W1981018711 hasIssue "1" @default.
- W1981018711 hasLocation W19810187111 @default.
- W1981018711 hasLocation W19810187112 @default.
- W1981018711 hasOpenAccess W1981018711 @default.
- W1981018711 hasPrimaryLocation W19810187111 @default.
- W1981018711 hasRelatedWork W1978748450 @default.
- W1981018711 hasRelatedWork W2090077964 @default.
- W1981018711 hasRelatedWork W2142619379 @default.
- W1981018711 hasRelatedWork W2150393939 @default.
- W1981018711 hasRelatedWork W2160900071 @default.
- W1981018711 hasRelatedWork W2473315520 @default.
- W1981018711 hasRelatedWork W2952038621 @default.
- W1981018711 hasRelatedWork W3147005653 @default.
- W1981018711 hasRelatedWork W4385933416 @default.
- W1981018711 hasRelatedWork W3021177496 @default.
- W1981018711 hasVolume "12" @default.
- W1981018711 isParatext "false" @default.
- W1981018711 isRetracted "false" @default.
- W1981018711 magId "1981018711" @default.
- W1981018711 workType "article" @default.