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- W1981019618 abstract "Inhibition of 3H-uridine incorporation into Candida albicans can be used as a sensitive index of phagocytic function because: 1) there is a linear correlation between uridine incorporation and yeast number; 2) phagocytic cells do not incorporate significant amounts of uridine in short term cultures; and 3) C. albicans replicating inside phagocytic cells does not take up uridine from culture medium. Appropriate conditions for measuring phagocytic capacity of human polymorphonuclear cells (PMNs) were 5 × 105C. albicans and 5 × 104 PMNs in 0.5 ml of medium containing 2.5% AB serum. This mixture was incubated for 30 min at 37°C. Aliquots were then transferred into microtiter wells and incubated for a further 60 min in the presence of 3H-uridine. Under these conditions PMN luucocytes from 25 healthy individuals caused suppression of uridine incorporation ranging from 33 to 75% (50 ± 12)." @default.
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- W1981019618 date "1977-01-01" @default.
- W1981019618 modified "2023-10-18" @default.
- W1981019618 title "Phagocytosis measured as inhibition of uridine uptake by Candida Albicans" @default.
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- W1981019618 doi "https://doi.org/10.1016/s0022-1759(97)90016-8" @default.
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