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- W1981068764 abstract "A polarographic method was optimised for the quantitative determination of metallothionein (MT) in whole body Gammarus locusta (Crustacea: Amphipoda). The identity of the MT-like proteins was examined using electrophoretic analysis (SDS-PAGE) and induction studies (water-borne exposures to copper). The MT-method was then used to examine changes in MT levels over the lifetime (juveniles, sub-adults and adults) of a laboratory population of G. locusta; copper (Cu) and zinc (Zn) body burden were also measured by flame atomic absorption spectrometry. MT was quantified by heat-denaturation of 30 000 g supernatants (to remove the bulk of non-MT proteins) followed by differential pulse polarography (DPP) to measure sulphydryl-group containing proteins. DPP proved to be a sensitive and reliable method to quantify heat-stable total sulphydryl-proteins. The majority of the latter was indicated to be MT from SDS-PAGE and induction studies. SDS-PAGE revealed a putative MT of 23 ± 0.6 kDa app. mol. wt., consistent wit..." @default.
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- W1981068764 date "2004-01-01" @default.
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- W1981068764 title "Quantification of metallothionein in whole body<i>gammarus locusta</i>(crustacea: amphipoda) using differential pulse polarography" @default.
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- W1981068764 doi "https://doi.org/10.1080/02772240410001665472" @default.
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