Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981085802> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1981085802 endingPage "6672" @default.
- W1981085802 startingPage "6668" @default.
- W1981085802 abstract "Twelve clones of origin-enriched sequences (ORS) isolated from early replicating monkey (CV-1) DNA were examined for transient episomal replication in transfected CV-1, COS-7, and HeLa cells. Plasmid DNA was isolated at time intervals after transfection and screened by the Dpn I resistance assay or by the bromodeoxyuridine substitution assay to differentiate between input and replicated DNA. We have identified four monkey ORS (ORS3, -8, -9, and -12) that can support plasmid replication in mammalian cells. This replication is carried out in a controlled and semiconservative manner characteristic of mammalian replicons. ORS replication was most efficient in HeLa cells. Electron microscopy showed ORS8 and ORS12 plasmids of the correct size with replication bubbles. Using a unique restriction site in ORS12, we have mapped the replication bubble within the monkey DNA sequence." @default.
- W1981085802 created "2016-06-24" @default.
- W1981085802 creator A5054297600 @default.
- W1981085802 creator A5060756067 @default.
- W1981085802 date "1987-10-01" @default.
- W1981085802 modified "2023-09-30" @default.
- W1981085802 title "Autonomous replication of plasmids bearing monkey DNA origin-enriched sequences." @default.
- W1981085802 cites W1775899900 @default.
- W1981085802 cites W1816498186 @default.
- W1981085802 cites W1829554488 @default.
- W1981085802 cites W1965372133 @default.
- W1981085802 cites W1967446284 @default.
- W1981085802 cites W1967842973 @default.
- W1981085802 cites W1972391171 @default.
- W1981085802 cites W1978617734 @default.
- W1981085802 cites W1980966767 @default.
- W1981085802 cites W2003473754 @default.
- W1981085802 cites W2004639615 @default.
- W1981085802 cites W2006864581 @default.
- W1981085802 cites W2033706348 @default.
- W1981085802 cites W2063450941 @default.
- W1981085802 cites W2071193905 @default.
- W1981085802 cites W2078575141 @default.
- W1981085802 cites W2084393103 @default.
- W1981085802 cites W2088167128 @default.
- W1981085802 cites W2098476705 @default.
- W1981085802 cites W2136347509 @default.
- W1981085802 cites W2152858220 @default.
- W1981085802 cites W2170817590 @default.
- W1981085802 cites W2315479748 @default.
- W1981085802 doi "https://doi.org/10.1073/pnas.84.19.6668" @default.
- W1981085802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/299144" @default.
- W1981085802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3477798" @default.
- W1981085802 hasPublicationYear "1987" @default.
- W1981085802 type Work @default.
- W1981085802 sameAs 1981085802 @default.
- W1981085802 citedByCount "71" @default.
- W1981085802 crossrefType "journal-article" @default.
- W1981085802 hasAuthorship W1981085802A5054297600 @default.
- W1981085802 hasAuthorship W1981085802A5060756067 @default.
- W1981085802 hasBestOaLocation W19810858021 @default.
- W1981085802 hasConcept C104317684 @default.
- W1981085802 hasConcept C104690221 @default.
- W1981085802 hasConcept C13052165 @default.
- W1981085802 hasConcept C153911025 @default.
- W1981085802 hasConcept C18103114 @default.
- W1981085802 hasConcept C22744801 @default.
- W1981085802 hasConcept C2778260397 @default.
- W1981085802 hasConcept C54009773 @default.
- W1981085802 hasConcept C54355233 @default.
- W1981085802 hasConcept C552990157 @default.
- W1981085802 hasConcept C58337764 @default.
- W1981085802 hasConcept C62112901 @default.
- W1981085802 hasConcept C73573662 @default.
- W1981085802 hasConcept C86803240 @default.
- W1981085802 hasConcept C98086919 @default.
- W1981085802 hasConceptScore W1981085802C104317684 @default.
- W1981085802 hasConceptScore W1981085802C104690221 @default.
- W1981085802 hasConceptScore W1981085802C13052165 @default.
- W1981085802 hasConceptScore W1981085802C153911025 @default.
- W1981085802 hasConceptScore W1981085802C18103114 @default.
- W1981085802 hasConceptScore W1981085802C22744801 @default.
- W1981085802 hasConceptScore W1981085802C2778260397 @default.
- W1981085802 hasConceptScore W1981085802C54009773 @default.
- W1981085802 hasConceptScore W1981085802C54355233 @default.
- W1981085802 hasConceptScore W1981085802C552990157 @default.
- W1981085802 hasConceptScore W1981085802C58337764 @default.
- W1981085802 hasConceptScore W1981085802C62112901 @default.
- W1981085802 hasConceptScore W1981085802C73573662 @default.
- W1981085802 hasConceptScore W1981085802C86803240 @default.
- W1981085802 hasConceptScore W1981085802C98086919 @default.
- W1981085802 hasIssue "19" @default.
- W1981085802 hasLocation W19810858021 @default.
- W1981085802 hasLocation W19810858022 @default.
- W1981085802 hasLocation W19810858023 @default.
- W1981085802 hasLocation W19810858024 @default.
- W1981085802 hasOpenAccess W1981085802 @default.
- W1981085802 hasPrimaryLocation W19810858021 @default.
- W1981085802 hasRelatedWork W1967078090 @default.
- W1981085802 hasRelatedWork W1981085802 @default.
- W1981085802 hasRelatedWork W1994187913 @default.
- W1981085802 hasRelatedWork W2002179331 @default.
- W1981085802 hasRelatedWork W2072283811 @default.
- W1981085802 hasRelatedWork W2095149823 @default.
- W1981085802 hasRelatedWork W2149065213 @default.
- W1981085802 hasRelatedWork W2158990839 @default.
- W1981085802 hasRelatedWork W2184669940 @default.
- W1981085802 hasRelatedWork W2238310462 @default.
- W1981085802 hasVolume "84" @default.
- W1981085802 isParatext "false" @default.
- W1981085802 isRetracted "false" @default.
- W1981085802 magId "1981085802" @default.
- W1981085802 workType "article" @default.