FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981124196> ?p ?o ?g. }

• W1981124196 endingPage "159" @default.
• W1981124196 startingPage "152" @default.
• W1981124196 abstract "Fibroblast growth factor receptor 4 (FGFR4) is a member of the FGFR family of receptor tyrosine kinases, and plays important roles in a variety of biological functions such as cell proliferation, differentiation, migration, angiogenesis, tissue repair, and tumorigenesis. The human FGFRs share a high degree of sequence homology between themselves, as well as with their murine homologs. Consequently, it has been suggested that it may be difficult to prepare monoclonal antibodies (MAbs) that are specific for the individual receptor types. In this communication, we report on the development and characterization of a panel of anti-human FGFR4 MAbs that were generated in mice using a rapid immunization protocol. Using a modified rapid immunization at multiple sites (RIMMS) protocol with the soluble extracellular domain of human FGFR4 (FGFR4-ECD), the immunized mice developed high levels of polyclonal IgG to the immunogen within 13 days of the first immunization. The lymph node cells isolated from the immunized animals were then fused with mouse myeloma cells for hybridoma generation. Use of an efficient hybridoma cloning protocol in combination with an ELISA screening procedure allowed for early identification of stable hybridomas secreting antihuman FGFR4 IgG. Several identified MAbs specifically reacted with the FGFR4 protein without binding to the other human isoforms (FGFR1, FGFR2, and FGFR3). As evaluated by BIAcore analysis, most anti-FGFR4 MAbs displayed high affinities (8.6 × 10−8 ∼ 3.9 × 10−10 M) to FGFR4. Furthermore, these MAbs were able to bind to FGFR4 expressed on human breast tumor cell lines MDA-MB-361 and MDA-MB-453. Taken together, the results demonstrate that the RIMMS strategy is an effective approach for generating class-switched, high-affinity MAbs in mice to evolutionarily conserved proteins such as human FGFR4. These MAbs may be useful tools for further investigation of the biological functions and pathological roles of human FGFR4." @default.
• W1981124196 created "2016-06-24" @default.
• W1981124196 creator A5003449723 @default.
• W1981124196 creator A5013211928 @default.
• W1981124196 creator A5019504883 @default.
• W1981124196 creator A5021997539 @default.
• W1981124196 creator A5044034230 @default.
• W1981124196 creator A5066061488 @default.
• W1981124196 creator A5071469941 @default.
• W1981124196 creator A5079346370 @default.
• W1981124196 creator A5086931916 @default.
• W1981124196 creator A5089402840 @default.
• W1981124196 date "2005-06-01" @default.
• W1981124196 modified "2023-09-25" @default.
• W1981124196 title "Generation and Characterization of a Panel of Monoclonal Antibodies Specific for Human Fibroblast Growth Factor Receptor 4 (FGFR4)" @default.
• W1981124196 cites W1493964952 @default.
• W1981124196 cites W1574432799 @default.
• W1981124196 cites W1799346688 @default.
• W1981124196 cites W1967013447 @default.
• W1981124196 cites W1977886818 @default.
• W1981124196 cites W1997276913 @default.
• W1981124196 cites W2002000962 @default.
• W1981124196 cites W2003632139 @default.
• W1981124196 cites W2005630069 @default.
• W1981124196 cites W2012677818 @default.
• W1981124196 cites W2021458499 @default.
• W1981124196 cites W2025079102 @default.
• W1981124196 cites W2032719221 @default.
• W1981124196 cites W2036895735 @default.
• W1981124196 cites W2037490831 @default.
• W1981124196 cites W2042055907 @default.
• W1981124196 cites W2050798682 @default.
• W1981124196 cites W2058212333 @default.
• W1981124196 cites W2063844198 @default.
• W1981124196 cites W2067676232 @default.
• W1981124196 cites W2072303535 @default.
• W1981124196 cites W2086905843 @default.
• W1981124196 cites W2113530505 @default.
• W1981124196 cites W2116264090 @default.
• W1981124196 cites W2138178479 @default.
• W1981124196 cites W2151953516 @default.
• W1981124196 cites W2314632338 @default.
• W1981124196 cites W4254460460 @default.
• W1981124196 doi "https://doi.org/10.1089/hyb.2005.24.152" @default.
• W1981124196 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15943563" @default.
• W1981124196 hasPublicationYear "2005" @default.
• W1981124196 type Work @default.
• W1981124196 sameAs 1981124196 @default.
• W1981124196 citedByCount "9" @default.
• W1981124196 countsByYear W19811241962012 @default.
• W1981124196 countsByYear W19811241962013 @default.
• W1981124196 countsByYear W19811241962014 @default.
• W1981124196 countsByYear W19811241962015 @default.
• W1981124196 crossrefType "journal-article" @default.
• W1981124196 hasAuthorship W1981124196A5003449723 @default.
• W1981124196 hasAuthorship W1981124196A5013211928 @default.
• W1981124196 hasAuthorship W1981124196A5019504883 @default.
• W1981124196 hasAuthorship W1981124196A5021997539 @default.
• W1981124196 hasAuthorship W1981124196A5044034230 @default.
• W1981124196 hasAuthorship W1981124196A5066061488 @default.
• W1981124196 hasAuthorship W1981124196A5071469941 @default.
• W1981124196 hasAuthorship W1981124196A5079346370 @default.
• W1981124196 hasAuthorship W1981124196A5086931916 @default.
• W1981124196 hasAuthorship W1981124196A5089402840 @default.
• W1981124196 hasConcept C101544691 @default.
• W1981124196 hasConcept C153911025 @default.
• W1981124196 hasConcept C159654299 @default.
• W1981124196 hasConcept C170493617 @default.
• W1981124196 hasConcept C203014093 @default.
• W1981124196 hasConcept C502942594 @default.
• W1981124196 hasConcept C542903549 @default.
• W1981124196 hasConcept C55493867 @default.
• W1981124196 hasConcept C66400584 @default.
• W1981124196 hasConcept C74373430 @default.
• W1981124196 hasConcept C82867764 @default.
• W1981124196 hasConcept C86803240 @default.
• W1981124196 hasConceptScore W1981124196C101544691 @default.
• W1981124196 hasConceptScore W1981124196C153911025 @default.
• W1981124196 hasConceptScore W1981124196C159654299 @default.
• W1981124196 hasConceptScore W1981124196C170493617 @default.
• W1981124196 hasConceptScore W1981124196C203014093 @default.
• W1981124196 hasConceptScore W1981124196C502942594 @default.
• W1981124196 hasConceptScore W1981124196C542903549 @default.
• W1981124196 hasConceptScore W1981124196C55493867 @default.
• W1981124196 hasConceptScore W1981124196C66400584 @default.
• W1981124196 hasConceptScore W1981124196C74373430 @default.
• W1981124196 hasConceptScore W1981124196C82867764 @default.
• W1981124196 hasConceptScore W1981124196C86803240 @default.
• W1981124196 hasIssue "3" @default.
• W1981124196 hasLocation W19811241961 @default.
• W1981124196 hasLocation W19811241962 @default.
• W1981124196 hasOpenAccess W1981124196 @default.
• W1981124196 hasPrimaryLocation W19811241961 @default.
• W1981124196 hasRelatedWork W1979761296 @default.
• W1981124196 hasRelatedWork W1990357663 @default.
• W1981124196 hasRelatedWork W2097580373 @default.
• W1981124196 hasRelatedWork W2107959852 @default.
• W1981124196 hasRelatedWork W2135949891 @default.

• next