FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981176722> ?p ?o ?g. }

• W1981176722 endingPage "368" @default.
• W1981176722 startingPage "359" @default.
• W1981176722 abstract "Pokeweed antiviral protein (PAP) from the leaves of the pokeweed plant, Phytolacca americana, is a naturally occurring single-chain ribosome-inactivating protein, which catalytically inactivates both prokaryotic and eukaryotic ribosomes. The therapeutic potential of PAP has gained considerable interest in recent years due to the clinical use of native PAP as the active moiety of immunoconjugates against cancer and AIDS. The clinical use of native PAP is limited due to inherent difficulties in obtaining sufficient quantities of a homogenously pure and active PAP preparation with minimal batch to batch variability from its natural source. Previous methods for expression of recombinant PAP in yeast, transgenic plants and Escherichia coli have resulted in either unacceptably low yields or were too toxic to the host system. Here, we report a successful strategy which allows high level expression of PAP as inclusion bodies in E. coli. Purification of refolded recombinant protein from solubilized inclusion bodies by size-exclusion chromatography yielded biologically active recombinant PAP (final yield: 10 to 12 mg/L). The ribosome depurinating in vitro N-glycosidase activity and cellular anti-HIV activity of recombinant PAP were comparable to those of the native PAP. This expression and purification system makes it possible to obtain sufficient quantities of biologically active and homogenous recombinant PAP sufficient to carry out advanced clinical trials. To our knowledge, this is the first large-scale expression and purification of biologically active recombinant PAP from E. coli." @default.
• W1981176722 created "2016-06-24" @default.
• W1981176722 creator A5006516910 @default.
• W1981176722 creator A5025576364 @default.
• W1981176722 creator A5028322615 @default.
• W1981176722 creator A5038351786 @default.
• W1981176722 creator A5046176431 @default.
• W1981176722 creator A5091381226 @default.
• W1981176722 date "1999-07-01" @default.
• W1981176722 modified "2023-09-26" @default.
• W1981176722 title "High-Level Expression and Purification of Biologically Active Recombinant Pokeweed Antiviral Protein" @default.
• W1981176722 cites W1482431053 @default.
• W1981176722 cites W1577035491 @default.
• W1981176722 cites W183993696 @default.
• W1981176722 cites W1982755822 @default.
• W1981176722 cites W1985067074 @default.
• W1981176722 cites W1988473696 @default.
• W1981176722 cites W1996829946 @default.
• W1981176722 cites W1999297279 @default.
• W1981176722 cites W2004834642 @default.
• W1981176722 cites W2006140229 @default.
• W1981176722 cites W2008291255 @default.
• W1981176722 cites W2022286725 @default.
• W1981176722 cites W2027148086 @default.
• W1981176722 cites W2039025340 @default.
• W1981176722 cites W2049315807 @default.
• W1981176722 cites W2055213393 @default.
• W1981176722 cites W2058390382 @default.
• W1981176722 cites W2060399130 @default.
• W1981176722 cites W2076823602 @default.
• W1981176722 cites W2101108802 @default.
• W1981176722 cites W2101963388 @default.
• W1981176722 cites W2110745460 @default.
• W1981176722 cites W2124708023 @default.
• W1981176722 cites W2158754011 @default.
• W1981176722 cites W2164860217 @default.
• W1981176722 doi "https://doi.org/10.1006/prep.1999.1084" @default.
• W1981176722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10419833" @default.
• W1981176722 hasPublicationYear "1999" @default.
• W1981176722 type Work @default.
• W1981176722 sameAs 1981176722 @default.
• W1981176722 citedByCount "32" @default.
• W1981176722 countsByYear W19811767222012 @default.
• W1981176722 countsByYear W19811767222013 @default.
• W1981176722 countsByYear W19811767222014 @default.
• W1981176722 countsByYear W19811767222015 @default.
• W1981176722 countsByYear W19811767222016 @default.
• W1981176722 countsByYear W19811767222020 @default.
• W1981176722 crossrefType "journal-article" @default.
• W1981176722 hasAuthorship W1981176722A5006516910 @default.
• W1981176722 hasAuthorship W1981176722A5025576364 @default.
• W1981176722 hasAuthorship W1981176722A5028322615 @default.
• W1981176722 hasAuthorship W1981176722A5038351786 @default.
• W1981176722 hasAuthorship W1981176722A5046176431 @default.
• W1981176722 hasAuthorship W1981176722A5091381226 @default.
• W1981176722 hasConcept C104317684 @default.
• W1981176722 hasConcept C116073593 @default.
• W1981176722 hasConcept C123894998 @default.
• W1981176722 hasConcept C141231307 @default.
• W1981176722 hasConcept C153911025 @default.
• W1981176722 hasConcept C202751555 @default.
• W1981176722 hasConcept C203750385 @default.
• W1981176722 hasConcept C2776746152 @default.
• W1981176722 hasConcept C2776781592 @default.
• W1981176722 hasConcept C2779222958 @default.
• W1981176722 hasConcept C2780128948 @default.
• W1981176722 hasConcept C30417494 @default.
• W1981176722 hasConcept C40767141 @default.
• W1981176722 hasConcept C547475151 @default.
• W1981176722 hasConcept C55493867 @default.
• W1981176722 hasConcept C67705224 @default.
• W1981176722 hasConcept C86803240 @default.
• W1981176722 hasConcept C88478588 @default.
• W1981176722 hasConceptScore W1981176722C104317684 @default.
• W1981176722 hasConceptScore W1981176722C116073593 @default.
• W1981176722 hasConceptScore W1981176722C123894998 @default.
• W1981176722 hasConceptScore W1981176722C141231307 @default.
• W1981176722 hasConceptScore W1981176722C153911025 @default.
• W1981176722 hasConceptScore W1981176722C202751555 @default.
• W1981176722 hasConceptScore W1981176722C203750385 @default.
• W1981176722 hasConceptScore W1981176722C2776746152 @default.
• W1981176722 hasConceptScore W1981176722C2776781592 @default.
• W1981176722 hasConceptScore W1981176722C2779222958 @default.
• W1981176722 hasConceptScore W1981176722C2780128948 @default.
• W1981176722 hasConceptScore W1981176722C30417494 @default.
• W1981176722 hasConceptScore W1981176722C40767141 @default.
• W1981176722 hasConceptScore W1981176722C547475151 @default.
• W1981176722 hasConceptScore W1981176722C55493867 @default.
• W1981176722 hasConceptScore W1981176722C67705224 @default.
• W1981176722 hasConceptScore W1981176722C86803240 @default.
• W1981176722 hasConceptScore W1981176722C88478588 @default.
• W1981176722 hasIssue "2" @default.
• W1981176722 hasLocation W19811767221 @default.
• W1981176722 hasLocation W19811767222 @default.
• W1981176722 hasOpenAccess W1981176722 @default.
• W1981176722 hasPrimaryLocation W19811767221 @default.
• W1981176722 hasRelatedWork W1432577577 @default.
• W1981176722 hasRelatedWork W1981176722 @default.

• next