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- W1981240254 abstract "Conventional confocal laser scanning microscopy uses continuous wave visible or UV lasers for one-photon excitation and imaging. These instruments are now enhanced with pulsed infrared lasers allowing two-photon excitation. With the appropriate technical setup two-photon excitation may be used for intensity imaging or for lifetime imaging. Both of these techniques have specific advantages if compared with one-photon excitation. Despite this fact, to date mainly one-photon excitation has been employed in microbial ecology. In this review the potential of two-photon intensity and lifetime imaging is discussed, using appropriate examples from cell biology as well as the first reports in microbiology." @default.
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- W1981240254 date "2004-01-01" @default.
- W1981240254 modified "2023-10-03" @default.
- W1981240254 title "Two-Photon Imaging for Studying the Microbial Ecology of Biofilm Systems" @default.
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- W1981240254 doi "https://doi.org/10.1264/jsme2.19.1" @default.
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