FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1981249456> ?p ?o ?g. }

• W1981249456 endingPage "268" @default.
• W1981249456 startingPage "255" @default.
• W1981249456 abstract "During the crawling movements of non-muscle cells, myosin II-containing structures assemble and disassemble with a high degree of spatial and temporal heterogeneity. In order to understand how this is controlled, we examined factors that influence the association of myosin II with detergent-resistant cytoskeletons of cultured endothelial cells. Treatment of cells with 0.05% Triton X-100 in an actin-stabilizing buffer released ∼42% of the myosin II from the cytoplasm. Most remaining myosin II was dissociated from the cytoskeleton by treatment with ATP or AMPPNP, but not ADP, suggesting that myosin II is retained as ATP-sensitive filaments or via rigor-like binding to F-actin. Disruption of actin filaments with cytochalasin or latrunculin prior to detergent permeabilization sharply decreased the amount of myosin II retained, suggesting the latter type of association. Because phosphorylation of myosin II affects filament assembly and actin binding in vitro, phosphorylation levels in soluble and cytoskeletal myosin II were measured. Phosphorylation of myosin heavy chains was not significantly different between the two fractions, but regulatory light chains of cytoskeletal myosin II were 5 times more phosphorylated than in soluble myosin II. Tryptic-peptide mapping showed that cytoskeletal light chains were phosphorylated predominantly at serine 19/threonine 18, which regulates myosin II assembly in vitro, whereas soluble light chains were not phosphorylated or were phosphorylated at threonine 9. Treating cells with the kinase inhibitor, staurosporine, prior to permeabilization decreased light-chain phosphorylation with concomitant reduction in myosin retention. These observations suggest that assembly of myosin II into cytoskeletal structures, where it can generate and resist forces, is regulated in vivo by phosphorylation of myosin light chains at serine 19/threonine 18. Cell Motil. Cytoskeleton 43:255–268, 1999. © 1999 Wiley-Liss, Inc." @default.
• W1981249456 created "2016-06-24" @default.
• W1981249456 creator A5031782254 @default.
• W1981249456 creator A5058942816 @default.
• W1981249456 date "1999-01-01" @default.
• W1981249456 modified "2023-09-28" @default.
• W1981249456 title "Regulatory light chain phosphorylation and the assembly of myosin II into the cytoskeleton of microcapillary endothelial cells" @default.
• W1981249456 cites W1522733018 @default.
• W1981249456 cites W1550857893 @default.
• W1981249456 cites W1976138565 @default.
• W1981249456 cites W1981255639 @default.
• W1981249456 cites W1981643167 @default.
• W1981249456 cites W1989482650 @default.
• W1981249456 cites W1990103360 @default.
• W1981249456 cites W1995283111 @default.
• W1981249456 cites W1996368518 @default.
• W1981249456 cites W1999469923 @default.
• W1981249456 cites W2004112317 @default.
• W1981249456 cites W2008544686 @default.
• W1981249456 cites W2012652910 @default.
• W1981249456 cites W2013622856 @default.
• W1981249456 cites W2019741069 @default.
• W1981249456 cites W2021991370 @default.
• W1981249456 cites W2023382138 @default.
• W1981249456 cites W2025353360 @default.
• W1981249456 cites W2033401525 @default.
• W1981249456 cites W2038551087 @default.
• W1981249456 cites W2047886675 @default.
• W1981249456 cites W2050277545 @default.
• W1981249456 cites W2050936797 @default.
• W1981249456 cites W2066256434 @default.
• W1981249456 cites W2084640600 @default.
• W1981249456 cites W2085849675 @default.
• W1981249456 cites W2086176469 @default.
• W1981249456 cites W2088603002 @default.
• W1981249456 cites W2089299883 @default.
• W1981249456 cites W2092222240 @default.
• W1981249456 cites W2094304910 @default.
• W1981249456 cites W2106483065 @default.
• W1981249456 cites W2111998377 @default.
• W1981249456 cites W2119632652 @default.
• W1981249456 cites W2122067618 @default.
• W1981249456 cites W2122088733 @default.
• W1981249456 cites W2140319446 @default.
• W1981249456 cites W2146318623 @default.
• W1981249456 cites W2149449829 @default.
• W1981249456 cites W2150102283 @default.
• W1981249456 cites W2155461712 @default.
• W1981249456 cites W2159578875 @default.
• W1981249456 cites W2166228642 @default.
• W1981249456 cites W2226885842 @default.
• W1981249456 cites W2285795908 @default.
• W1981249456 cites W2336469222 @default.
• W1981249456 cites W2471466228 @default.
• W1981249456 doi "https://doi.org/10.1002/(sici)1097-0169(1999)43:3<255::aid-cm8>3.0.co;2-t" @default.
• W1981249456 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10401581" @default.
• W1981249456 hasPublicationYear "1999" @default.
• W1981249456 type Work @default.
• W1981249456 sameAs 1981249456 @default.
• W1981249456 citedByCount "25" @default.
• W1981249456 countsByYear W19812494562014 @default.
• W1981249456 countsByYear W19812494562015 @default.
• W1981249456 countsByYear W19812494562017 @default.
• W1981249456 countsByYear W19812494562018 @default.
• W1981249456 countsByYear W19812494562019 @default.
• W1981249456 crossrefType "journal-article" @default.
• W1981249456 hasAuthorship W1981249456A5031782254 @default.
• W1981249456 hasAuthorship W1981249456A5058942816 @default.
• W1981249456 hasConcept C11960822 @default.
• W1981249456 hasConcept C125705527 @default.
• W1981249456 hasConcept C142669718 @default.
• W1981249456 hasConcept C1491633281 @default.
• W1981249456 hasConcept C189040839 @default.
• W1981249456 hasConcept C55493867 @default.
• W1981249456 hasConcept C6997183 @default.
• W1981249456 hasConcept C8035138 @default.
• W1981249456 hasConcept C86803240 @default.
• W1981249456 hasConcept C95444343 @default.
• W1981249456 hasConceptScore W1981249456C11960822 @default.
• W1981249456 hasConceptScore W1981249456C125705527 @default.
• W1981249456 hasConceptScore W1981249456C142669718 @default.
• W1981249456 hasConceptScore W1981249456C1491633281 @default.
• W1981249456 hasConceptScore W1981249456C189040839 @default.
• W1981249456 hasConceptScore W1981249456C55493867 @default.
• W1981249456 hasConceptScore W1981249456C6997183 @default.
• W1981249456 hasConceptScore W1981249456C8035138 @default.
• W1981249456 hasConceptScore W1981249456C86803240 @default.
• W1981249456 hasConceptScore W1981249456C95444343 @default.
• W1981249456 hasIssue "3" @default.
• W1981249456 hasLocation W19812494561 @default.
• W1981249456 hasLocation W19812494562 @default.
• W1981249456 hasOpenAccess W1981249456 @default.
• W1981249456 hasPrimaryLocation W19812494561 @default.
• W1981249456 hasRelatedWork W1970793486 @default.
• W1981249456 hasRelatedWork W2002131028 @default.
• W1981249456 hasRelatedWork W2006124456 @default.
• W1981249456 hasRelatedWork W2024879277 @default.
• W1981249456 hasRelatedWork W2033472087 @default.

• next