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- W1981257515 abstract "Novel amphiphiles which carry many mannose residues as side chains were prepared by telomerization of N-methacryloylaminopropyl d-mannopyranoside (α:β = 20:1), N-methacryloylaminohexyl d-mannopyranoside (α:β = 20:1), or 3-(2-methacryloylaminoethylthio)propyl d-mannopyranoside (α:β = 4:1) using a lipophilic radical initiator. The mannose-carrying amphiphiles incorporated in liposomes were recognized by a lectin from Canavalia ensiformis (Con A), which was proven by the increase in turbidity of the liposome suspension after mixing with Con A. The interaction between sugar residues on the liposome surface and the lectin was largely affected by the degree of polymerization (DP) and the surface density of the amphiphile in the liposomes. The distance between the sugar residues and the polymer main chain did not affect the specific recognition by the lectin significantly in the liposome system, whereas it appreciably affected the recognition in the water-soluble polymer system. The association constants (Ka) of the amphiphiles (DP ≃ 18) with Con A (0.3−2.2 × 106 M-1 at 25 °C) were much larger than that of α-methyl d-mannopyranoside (8.2 × 103 M-1) due to the “cluster effect ”. The positive entropy change (20−52 J/mol K) for the binding of Con A to mannose residues on the liposome surface showed that the recognition in the liposome system was largely promoted by the release of water molecules from both the sugar residues on the liposome surface and the binding site of Con A." @default.
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- W1981257515 date "1999-04-22" @default.
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- W1981257515 title "Recognition of Novel Amphiphiles with Many Pendent Mannose Residues by Con A" @default.
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- W1981257515 doi "https://doi.org/10.1021/bc980083x" @default.
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